Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS

The existence of glucose conjugates of fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB(2) and FB(3) bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B(2) (NDfrc-FB(2)) and N-(1-deoxy-d-fructos-1-...

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Autores principales: Matsuo, Yosuke, Takahara, Kentaro, Sago, Yuki, Kushiro, Masayo, Nagashima, Hitoshi, Nakagawa, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591641/
https://www.ncbi.nlm.nih.gov/pubmed/26389955
http://dx.doi.org/10.3390/toxins7093700
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author Matsuo, Yosuke
Takahara, Kentaro
Sago, Yuki
Kushiro, Masayo
Nagashima, Hitoshi
Nakagawa, Hiroyuki
author_facet Matsuo, Yosuke
Takahara, Kentaro
Sago, Yuki
Kushiro, Masayo
Nagashima, Hitoshi
Nakagawa, Hiroyuki
author_sort Matsuo, Yosuke
collection PubMed
description The existence of glucose conjugates of fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB(2) and FB(3) bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B(2) (NDfrc-FB(2)) and N-(1-deoxy-d-fructos-1-yl) fumonisin B(3) (NDfrc-FB(3)) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB(2) and NDfrc-FB(3) with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB(2) and FB(3) molecules occurred to their primary amine residues.
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spelling pubmed-45916412015-10-05 Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS Matsuo, Yosuke Takahara, Kentaro Sago, Yuki Kushiro, Masayo Nagashima, Hitoshi Nakagawa, Hiroyuki Toxins (Basel) Article The existence of glucose conjugates of fumonisin B(2) (FB(2)) and fumonisin B(3) (FB(3)) in corn powder was confirmed for the first time. These “bound-fumonisins” (FB(2) and FB(3) bound to glucose) were identified as N-(1-deoxy-d-fructos-1-yl) fumonisin B(2) (NDfrc-FB(2)) and N-(1-deoxy-d-fructos-1-yl) fumonisin B(3) (NDfrc-FB(3)) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB(2) and NDfrc-FB(3) with the o-phthalaldehyde (OPA) reagent also supported that d-glucose binding to FB(2) and FB(3) molecules occurred to their primary amine residues. MDPI 2015-09-16 /pmc/articles/PMC4591641/ /pubmed/26389955 http://dx.doi.org/10.3390/toxins7093700 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsuo, Yosuke
Takahara, Kentaro
Sago, Yuki
Kushiro, Masayo
Nagashima, Hitoshi
Nakagawa, Hiroyuki
Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title_full Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title_fullStr Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title_full_unstemmed Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title_short Detection of N-(1-deoxy-d-fructos-1-yl) Fumonisins B(2) and B(3) in Corn by High-Resolution LC-Orbitrap MS
title_sort detection of n-(1-deoxy-d-fructos-1-yl) fumonisins b(2) and b(3) in corn by high-resolution lc-orbitrap ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591641/
https://www.ncbi.nlm.nih.gov/pubmed/26389955
http://dx.doi.org/10.3390/toxins7093700
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