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Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel
The intense pain induced by scorpion sting is a frequent clinical manifestation. To date, there is no established protocol with significant efficacy to alleviate the pain induced by scorpion envenomation. One of the important reasons is that, little information on pain-inducing compound from scorpio...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591660/ https://www.ncbi.nlm.nih.gov/pubmed/26389953 http://dx.doi.org/10.3390/toxins7093671 |
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author | Hakim, Md Abdul Jiang, Wenbin Luo, Lei Li, Bowen Yang, Shilong Song, Yuzhu Lai, Ren |
author_facet | Hakim, Md Abdul Jiang, Wenbin Luo, Lei Li, Bowen Yang, Shilong Song, Yuzhu Lai, Ren |
author_sort | Hakim, Md Abdul |
collection | PubMed |
description | The intense pain induced by scorpion sting is a frequent clinical manifestation. To date, there is no established protocol with significant efficacy to alleviate the pain induced by scorpion envenomation. One of the important reasons is that, little information on pain-inducing compound from scorpion venoms is available. Here, a pain-inducing peptide (BmP01) has been identified and characterized from the venoms of scorpion (Mesobuthus martensii). In an animal model, intraplantar injection of BmP01 in mouse hind paw showed significant acute pain in wild type (WT) mice but not in TRPV1 knock-out (TRPV1 KO) mice during 30 min recording. BmP01 evoked currents in WT dorsal root ganglion (DRG) neurons but had no effect on DRG neurons of TRPV1 KO mice. Furthermore, BmP01 evoked currents on TRPV1-expressed HEK293T cells, but not on HEK293T cells without TRPV1. These results suggest that (1) BmP01 is one of the pain-inducing agents in scorpion venoms; and (2) BmP01 induces pain by acting on TRPV1. To our knowledge, this is the first report about a scorpion toxin that produces pain by targeting TRPV1. Identification of a pain-inducing compound may facilitate treating pain induced by scorpion envenomation. |
format | Online Article Text |
id | pubmed-4591660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45916602015-10-05 Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel Hakim, Md Abdul Jiang, Wenbin Luo, Lei Li, Bowen Yang, Shilong Song, Yuzhu Lai, Ren Toxins (Basel) Article The intense pain induced by scorpion sting is a frequent clinical manifestation. To date, there is no established protocol with significant efficacy to alleviate the pain induced by scorpion envenomation. One of the important reasons is that, little information on pain-inducing compound from scorpion venoms is available. Here, a pain-inducing peptide (BmP01) has been identified and characterized from the venoms of scorpion (Mesobuthus martensii). In an animal model, intraplantar injection of BmP01 in mouse hind paw showed significant acute pain in wild type (WT) mice but not in TRPV1 knock-out (TRPV1 KO) mice during 30 min recording. BmP01 evoked currents in WT dorsal root ganglion (DRG) neurons but had no effect on DRG neurons of TRPV1 KO mice. Furthermore, BmP01 evoked currents on TRPV1-expressed HEK293T cells, but not on HEK293T cells without TRPV1. These results suggest that (1) BmP01 is one of the pain-inducing agents in scorpion venoms; and (2) BmP01 induces pain by acting on TRPV1. To our knowledge, this is the first report about a scorpion toxin that produces pain by targeting TRPV1. Identification of a pain-inducing compound may facilitate treating pain induced by scorpion envenomation. MDPI 2015-09-14 /pmc/articles/PMC4591660/ /pubmed/26389953 http://dx.doi.org/10.3390/toxins7093671 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hakim, Md Abdul Jiang, Wenbin Luo, Lei Li, Bowen Yang, Shilong Song, Yuzhu Lai, Ren Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title | Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title_full | Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title_fullStr | Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title_full_unstemmed | Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title_short | Scorpion Toxin, BmP01, Induces Pain by Targeting TRPV1 Channel |
title_sort | scorpion toxin, bmp01, induces pain by targeting trpv1 channel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591660/ https://www.ncbi.nlm.nih.gov/pubmed/26389953 http://dx.doi.org/10.3390/toxins7093671 |
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