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A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes

A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf)...

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Autores principales: Hopper, Daniel W, Ghan, Ryan, Cramer, Grant R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591676/
https://www.ncbi.nlm.nih.gov/pubmed/26504528
http://dx.doi.org/10.1038/hortres.2014.2
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author Hopper, Daniel W
Ghan, Ryan
Cramer, Grant R
author_facet Hopper, Daniel W
Ghan, Ryan
Cramer, Grant R
author_sort Hopper, Daniel W
collection PubMed
description A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.
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spelling pubmed-45916762015-10-26 A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes Hopper, Daniel W Ghan, Ryan Cramer, Grant R Hortic Res Article A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration. Nature Publishing Group 2014-01-22 /pmc/articles/PMC4591676/ /pubmed/26504528 http://dx.doi.org/10.1038/hortres.2014.2 Text en Copyright © 2013 Nanjing Agricultural University http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Hopper, Daniel W
Ghan, Ryan
Cramer, Grant R
A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title_full A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title_fullStr A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title_full_unstemmed A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title_short A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
title_sort rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591676/
https://www.ncbi.nlm.nih.gov/pubmed/26504528
http://dx.doi.org/10.1038/hortres.2014.2
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