Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification

Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heate...

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Detalles Bibliográficos
Autores principales: Shah, Kamal G., Guelig, Dylan, Diesburg, Steven, Buser, Joshua, Burton, Robert, LaBarre, Paul, Richards-Kortum, Rebecca, Weigl, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591995/
https://www.ncbi.nlm.nih.gov/pubmed/26430883
http://dx.doi.org/10.1371/journal.pone.0139449
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author Shah, Kamal G.
Guelig, Dylan
Diesburg, Steven
Buser, Joshua
Burton, Robert
LaBarre, Paul
Richards-Kortum, Rebecca
Weigl, Bernhard
author_facet Shah, Kamal G.
Guelig, Dylan
Diesburg, Steven
Buser, Joshua
Burton, Robert
LaBarre, Paul
Richards-Kortum, Rebecca
Weigl, Bernhard
author_sort Shah, Kamal G.
collection PubMed
description Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters.
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spelling pubmed-45919952015-10-09 Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification Shah, Kamal G. Guelig, Dylan Diesburg, Steven Buser, Joshua Burton, Robert LaBarre, Paul Richards-Kortum, Rebecca Weigl, Bernhard PLoS One Research Article Previous chemical heater designs for isothermal nucleic acid amplification have been based on solid-liquid phase transition, but using this approach, developers have identified design challenges en route to developing a low-cost, disposable device. Here, we demonstrate the feasibility of a new heater configuration suitable for isothermal amplification in which one reactant of an exothermic reaction is a liquid-gas phase-change material, thereby eliminating the need for a separate phase-change compartment. This design offers potentially enhanced performance and energy density compared to other chemical and electric heaters. Public Library of Science 2015-10-02 /pmc/articles/PMC4591995/ /pubmed/26430883 http://dx.doi.org/10.1371/journal.pone.0139449 Text en © 2015 Shah et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shah, Kamal G.
Guelig, Dylan
Diesburg, Steven
Buser, Joshua
Burton, Robert
LaBarre, Paul
Richards-Kortum, Rebecca
Weigl, Bernhard
Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title_full Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title_fullStr Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title_full_unstemmed Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title_short Design of a New Type of Compact Chemical Heater for Isothermal Nucleic Acid Amplification
title_sort design of a new type of compact chemical heater for isothermal nucleic acid amplification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4591995/
https://www.ncbi.nlm.nih.gov/pubmed/26430883
http://dx.doi.org/10.1371/journal.pone.0139449
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