A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica

A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To ch...

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Autores principales: Chen, Jinhuan, Zhang, Dongzhi, Zhang, Chong, Xia, Xinli, Yin, Weilun, Tian, Qianqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592019/
https://www.ncbi.nlm.nih.gov/pubmed/26431530
http://dx.doi.org/10.1371/journal.pone.0139466
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author Chen, Jinhuan
Zhang, Dongzhi
Zhang, Chong
Xia, Xinli
Yin, Weilun
Tian, Qianqian
author_facet Chen, Jinhuan
Zhang, Dongzhi
Zhang, Chong
Xia, Xinli
Yin, Weilun
Tian, Qianqian
author_sort Chen, Jinhuan
collection PubMed
description A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.
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spelling pubmed-45920192015-10-09 A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica Chen, Jinhuan Zhang, Dongzhi Zhang, Chong Xia, Xinli Yin, Weilun Tian, Qianqian PLoS One Research Article A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar. Public Library of Science 2015-10-02 /pmc/articles/PMC4592019/ /pubmed/26431530 http://dx.doi.org/10.1371/journal.pone.0139466 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Jinhuan
Zhang, Dongzhi
Zhang, Chong
Xia, Xinli
Yin, Weilun
Tian, Qianqian
A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title_full A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title_fullStr A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title_full_unstemmed A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title_short A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica
title_sort putative pp2c-encoding gene negatively regulates aba signaling in populus euphratica
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592019/
https://www.ncbi.nlm.nih.gov/pubmed/26431530
http://dx.doi.org/10.1371/journal.pone.0139466
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