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Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong

BACKGROUND: A multicenter study was conducted to evaluate the diagnostic performance and the time to identifcation of the Verigene Blood Culture Test, the BC-GP and BC-GN assays, to identify both Gram-positive and Gram-negative bacteria and their drug resistance determinants directly from positive b...

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Autores principales: Siu, Gilman K. H., Chen, Jonathan H. K., Ng, T. K., Lee, Rodney A., Fung, Kitty S. C., To, Sabrina W. C., Wong, Barry K. C., Cheung, Sherman, Wong, Ivan W. F., Tam, Marble M. P., Lee, Swing S. W., Yam, W. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592242/
https://www.ncbi.nlm.nih.gov/pubmed/26431434
http://dx.doi.org/10.1371/journal.pone.0139728
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author Siu, Gilman K. H.
Chen, Jonathan H. K.
Ng, T. K.
Lee, Rodney A.
Fung, Kitty S. C.
To, Sabrina W. C.
Wong, Barry K. C.
Cheung, Sherman
Wong, Ivan W. F.
Tam, Marble M. P.
Lee, Swing S. W.
Yam, W. C.
author_facet Siu, Gilman K. H.
Chen, Jonathan H. K.
Ng, T. K.
Lee, Rodney A.
Fung, Kitty S. C.
To, Sabrina W. C.
Wong, Barry K. C.
Cheung, Sherman
Wong, Ivan W. F.
Tam, Marble M. P.
Lee, Swing S. W.
Yam, W. C.
author_sort Siu, Gilman K. H.
collection PubMed
description BACKGROUND: A multicenter study was conducted to evaluate the diagnostic performance and the time to identifcation of the Verigene Blood Culture Test, the BC-GP and BC-GN assays, to identify both Gram-positive and Gram-negative bacteria and their drug resistance determinants directly from positive blood cultures collected in Hong Kong. METHODS AND RESULTS: A total of 364 blood cultures were prospectively collected from four public hospitals, in which 114 and 250 cultures yielded Gram-positive and Gram-negative bacteria, and were tested with the BC-GP and BC-GN assay respectively. The overall identification agreement for Gram-positive and Gram-negative bacteria were 89.6% and 90.5% in monomicrobial cultures and 62.5% and 53.6% in polymicrobial cultures, respectively. The sensitivities for most genus/species achieved at least 80% except Enterococcus spp. (60%), K.oxytoca (0%), K.pneumoniae (69.2%), whereas the specificities for all targets ranged from 98.9% to 100%. Of note, 50% (7/14) cultures containing K.pneumoniae that were missed by the BC-GN assay were subsequently identified as K.variicola. Approximately 5.5% (20/364) cultures contained non-target organisms, of which Aeromonas spp. accounted for 25% and are of particular concern. For drug resistance determination, the Verigene test showed 100% sensitivity for identification of MRSA, VRE and carbapenem resistant Acinetobacter, and 84.4% for ESBL-producing Enterobacteriaceae based on the positive detection of mecA, vanA, bla (OXA) and bla (CTXM) respectively. CONCLUSION: Overall, the Verigene test provided acceptable accuracy for identification of bacteria and resistance markers with a range of turnaround time 40.5 to 99.2 h faster than conventional methods in our region.
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spelling pubmed-45922422015-10-09 Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong Siu, Gilman K. H. Chen, Jonathan H. K. Ng, T. K. Lee, Rodney A. Fung, Kitty S. C. To, Sabrina W. C. Wong, Barry K. C. Cheung, Sherman Wong, Ivan W. F. Tam, Marble M. P. Lee, Swing S. W. Yam, W. C. PLoS One Research Article BACKGROUND: A multicenter study was conducted to evaluate the diagnostic performance and the time to identifcation of the Verigene Blood Culture Test, the BC-GP and BC-GN assays, to identify both Gram-positive and Gram-negative bacteria and their drug resistance determinants directly from positive blood cultures collected in Hong Kong. METHODS AND RESULTS: A total of 364 blood cultures were prospectively collected from four public hospitals, in which 114 and 250 cultures yielded Gram-positive and Gram-negative bacteria, and were tested with the BC-GP and BC-GN assay respectively. The overall identification agreement for Gram-positive and Gram-negative bacteria were 89.6% and 90.5% in monomicrobial cultures and 62.5% and 53.6% in polymicrobial cultures, respectively. The sensitivities for most genus/species achieved at least 80% except Enterococcus spp. (60%), K.oxytoca (0%), K.pneumoniae (69.2%), whereas the specificities for all targets ranged from 98.9% to 100%. Of note, 50% (7/14) cultures containing K.pneumoniae that were missed by the BC-GN assay were subsequently identified as K.variicola. Approximately 5.5% (20/364) cultures contained non-target organisms, of which Aeromonas spp. accounted for 25% and are of particular concern. For drug resistance determination, the Verigene test showed 100% sensitivity for identification of MRSA, VRE and carbapenem resistant Acinetobacter, and 84.4% for ESBL-producing Enterobacteriaceae based on the positive detection of mecA, vanA, bla (OXA) and bla (CTXM) respectively. CONCLUSION: Overall, the Verigene test provided acceptable accuracy for identification of bacteria and resistance markers with a range of turnaround time 40.5 to 99.2 h faster than conventional methods in our region. Public Library of Science 2015-10-02 /pmc/articles/PMC4592242/ /pubmed/26431434 http://dx.doi.org/10.1371/journal.pone.0139728 Text en © 2015 Siu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Siu, Gilman K. H.
Chen, Jonathan H. K.
Ng, T. K.
Lee, Rodney A.
Fung, Kitty S. C.
To, Sabrina W. C.
Wong, Barry K. C.
Cheung, Sherman
Wong, Ivan W. F.
Tam, Marble M. P.
Lee, Swing S. W.
Yam, W. C.
Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title_full Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title_fullStr Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title_full_unstemmed Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title_short Performance Evaluation of the Verigene Gram-Positive and Gram-Negative Blood Culture Test for Direct Identification of Bacteria and Their Resistance Determinants from Positive Blood Cultures in Hong Kong
title_sort performance evaluation of the verigene gram-positive and gram-negative blood culture test for direct identification of bacteria and their resistance determinants from positive blood cultures in hong kong
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592242/
https://www.ncbi.nlm.nih.gov/pubmed/26431434
http://dx.doi.org/10.1371/journal.pone.0139728
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