Recognition Code of ZNF191(243-368) and Its Interaction with DNA

ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys(2)His(2) zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia co...

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Detalles Bibliográficos
Autores principales: Zhao, Dongxin, Huang, Zhongxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592708/
https://www.ncbi.nlm.nih.gov/pubmed/26457075
http://dx.doi.org/10.1155/2015/416751
Descripción
Sumario:ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys(2)His(2) zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21. The fusion protein GST-ZNF191(243-368) was expressed using this vector to investigate the protein-DNA binding reaction through an affinity selection strategy on the basis of the binding quality of the zinc finger domain. Results showed that ZNF191(243-368) can selectively bind with sequences and react with genes which contain an AGGG core. However, the recognition mechanism of Cys(2)His(2) zinc finger proteins to DNA warrants further investigation.

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