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Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection
Childhood malaria is a risk factor for disseminated infections with non-typhoidal Salmonella (NTS) in sub-Saharan Africa. While hemolytic anemia and an altered cytokine environment have been implicated in increased susceptibility to NTS, it is not known whether malaria affects resistance to intestin...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592952/ https://www.ncbi.nlm.nih.gov/pubmed/26434367 http://dx.doi.org/10.1038/srep14603 |
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author | Mooney, Jason P. Lokken, Kristen L. Byndloss, Mariana X. George, Michael D. Velazquez, Eric M. Faber, Franziska Butler, Brian P. Walker, Gregory T. Ali, Mohamed M. Potts, Rashaun Tiffany, Caitlin Ahmer, Brian M. M. Luckhart, Shirley Tsolis, Renée M. |
author_facet | Mooney, Jason P. Lokken, Kristen L. Byndloss, Mariana X. George, Michael D. Velazquez, Eric M. Faber, Franziska Butler, Brian P. Walker, Gregory T. Ali, Mohamed M. Potts, Rashaun Tiffany, Caitlin Ahmer, Brian M. M. Luckhart, Shirley Tsolis, Renée M. |
author_sort | Mooney, Jason P. |
collection | PubMed |
description | Childhood malaria is a risk factor for disseminated infections with non-typhoidal Salmonella (NTS) in sub-Saharan Africa. While hemolytic anemia and an altered cytokine environment have been implicated in increased susceptibility to NTS, it is not known whether malaria affects resistance to intestinal colonization with NTS. To address this question, we utilized a murine model of co-infection. Infection of mice with Plasmodium yoelii elicited infiltration of inflammatory macrophages and T cells into the intestinal mucosa and increased expression of inflammatory cytokines. These mucosal responses were also observed in germ-free mice, showing that they are independent of the resident microbiota. Remarkably, P. yoelii infection reduced colonization resistance of mice against S. enterica serotype Typhimurium. Further, 16S rRNA sequence analysis of the intestinal microbiota revealed marked changes in the community structure. Shifts in the microbiota increased susceptibility to intestinal colonization by S. Typhimurium, as demonstrated by microbiota reconstitution of germ-free mice. These results show that P. yoelii infection, via alterations to the microbial community in the intestine, decreases resistance to intestinal colonization with NTS. Further they raise the possibility that decreased colonization resistance may synergize with effects of malaria on systemic immunity to increase susceptibility to disseminated NTS infections. |
format | Online Article Text |
id | pubmed-4592952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45929522015-10-19 Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection Mooney, Jason P. Lokken, Kristen L. Byndloss, Mariana X. George, Michael D. Velazquez, Eric M. Faber, Franziska Butler, Brian P. Walker, Gregory T. Ali, Mohamed M. Potts, Rashaun Tiffany, Caitlin Ahmer, Brian M. M. Luckhart, Shirley Tsolis, Renée M. Sci Rep Article Childhood malaria is a risk factor for disseminated infections with non-typhoidal Salmonella (NTS) in sub-Saharan Africa. While hemolytic anemia and an altered cytokine environment have been implicated in increased susceptibility to NTS, it is not known whether malaria affects resistance to intestinal colonization with NTS. To address this question, we utilized a murine model of co-infection. Infection of mice with Plasmodium yoelii elicited infiltration of inflammatory macrophages and T cells into the intestinal mucosa and increased expression of inflammatory cytokines. These mucosal responses were also observed in germ-free mice, showing that they are independent of the resident microbiota. Remarkably, P. yoelii infection reduced colonization resistance of mice against S. enterica serotype Typhimurium. Further, 16S rRNA sequence analysis of the intestinal microbiota revealed marked changes in the community structure. Shifts in the microbiota increased susceptibility to intestinal colonization by S. Typhimurium, as demonstrated by microbiota reconstitution of germ-free mice. These results show that P. yoelii infection, via alterations to the microbial community in the intestine, decreases resistance to intestinal colonization with NTS. Further they raise the possibility that decreased colonization resistance may synergize with effects of malaria on systemic immunity to increase susceptibility to disseminated NTS infections. Nature Publishing Group 2015-10-05 /pmc/articles/PMC4592952/ /pubmed/26434367 http://dx.doi.org/10.1038/srep14603 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mooney, Jason P. Lokken, Kristen L. Byndloss, Mariana X. George, Michael D. Velazquez, Eric M. Faber, Franziska Butler, Brian P. Walker, Gregory T. Ali, Mohamed M. Potts, Rashaun Tiffany, Caitlin Ahmer, Brian M. M. Luckhart, Shirley Tsolis, Renée M. Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title | Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title_full | Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title_fullStr | Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title_full_unstemmed | Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title_short | Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection |
title_sort | inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal salmonella during concurrent malaria parasite infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592952/ https://www.ncbi.nlm.nih.gov/pubmed/26434367 http://dx.doi.org/10.1038/srep14603 |
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