Rapid low dose electron tomography using a direct electron detection camera

We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt seri...

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Detalles Bibliográficos
Autores principales: Migunov, Vadim, Ryll, Henning, Zhuge, Xiaodong, Simson, Martin, Strüder, Lothar, Batenburg, K. Joost, Houben, Lothar, Dunin-Borkowski, Rafal E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592966/
https://www.ncbi.nlm.nih.gov/pubmed/26434767
http://dx.doi.org/10.1038/srep14516
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author Migunov, Vadim
Ryll, Henning
Zhuge, Xiaodong
Simson, Martin
Strüder, Lothar
Batenburg, K. Joost
Houben, Lothar
Dunin-Borkowski, Rafal E.
author_facet Migunov, Vadim
Ryll, Henning
Zhuge, Xiaodong
Simson, Martin
Strüder, Lothar
Batenburg, K. Joost
Houben, Lothar
Dunin-Borkowski, Rafal E.
author_sort Migunov, Vadim
collection PubMed
description We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15–60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.
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spelling pubmed-45929662015-10-19 Rapid low dose electron tomography using a direct electron detection camera Migunov, Vadim Ryll, Henning Zhuge, Xiaodong Simson, Martin Strüder, Lothar Batenburg, K. Joost Houben, Lothar Dunin-Borkowski, Rafal E. Sci Rep Article We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15–60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution. Nature Publishing Group 2015-10-05 /pmc/articles/PMC4592966/ /pubmed/26434767 http://dx.doi.org/10.1038/srep14516 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Migunov, Vadim
Ryll, Henning
Zhuge, Xiaodong
Simson, Martin
Strüder, Lothar
Batenburg, K. Joost
Houben, Lothar
Dunin-Borkowski, Rafal E.
Rapid low dose electron tomography using a direct electron detection camera
title Rapid low dose electron tomography using a direct electron detection camera
title_full Rapid low dose electron tomography using a direct electron detection camera
title_fullStr Rapid low dose electron tomography using a direct electron detection camera
title_full_unstemmed Rapid low dose electron tomography using a direct electron detection camera
title_short Rapid low dose electron tomography using a direct electron detection camera
title_sort rapid low dose electron tomography using a direct electron detection camera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592966/
https://www.ncbi.nlm.nih.gov/pubmed/26434767
http://dx.doi.org/10.1038/srep14516
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