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Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis
Transcriptomes provide excellent foundational resources for mechanistic and evolutionary analyses of complex traits. We present a developmental transcriptome for the facultatively eusocial bee Megalopta genalis, which represents a potential transition point in the evolution of eusociality. A de novo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592995/ https://www.ncbi.nlm.nih.gov/pubmed/26276382 http://dx.doi.org/10.1534/g3.115.021261 |
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author | Jones, Beryl M. Wcislo, William T. Robinson, Gene E. |
author_facet | Jones, Beryl M. Wcislo, William T. Robinson, Gene E. |
author_sort | Jones, Beryl M. |
collection | PubMed |
description | Transcriptomes provide excellent foundational resources for mechanistic and evolutionary analyses of complex traits. We present a developmental transcriptome for the facultatively eusocial bee Megalopta genalis, which represents a potential transition point in the evolution of eusociality. A de novo transcriptome assembly of Megalopta genalis was generated using paired-end Illumina sequencing and the Trinity assembler. Males and females of all life stages were aligned to this transcriptome for analysis of gene expression profiles throughout development. Gene Ontology analysis indicates that stage-specific genes are involved in ion transport, cell–cell signaling, and metabolism. A number of distinct biological processes are upregulated in each life stage, and transitions between life stages involve shifts in dominant functional processes, including shifts from transcriptional regulation in embryos to metabolism in larvae, and increased lipid metabolism in adults. We expect that this transcriptome will provide a useful resource for future analyses to better understand the molecular basis of the evolution of eusociality and, more generally, phenotypic plasticity. |
format | Online Article Text |
id | pubmed-4592995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-45929952015-10-15 Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis Jones, Beryl M. Wcislo, William T. Robinson, Gene E. G3 (Bethesda) Investigations Transcriptomes provide excellent foundational resources for mechanistic and evolutionary analyses of complex traits. We present a developmental transcriptome for the facultatively eusocial bee Megalopta genalis, which represents a potential transition point in the evolution of eusociality. A de novo transcriptome assembly of Megalopta genalis was generated using paired-end Illumina sequencing and the Trinity assembler. Males and females of all life stages were aligned to this transcriptome for analysis of gene expression profiles throughout development. Gene Ontology analysis indicates that stage-specific genes are involved in ion transport, cell–cell signaling, and metabolism. A number of distinct biological processes are upregulated in each life stage, and transitions between life stages involve shifts in dominant functional processes, including shifts from transcriptional regulation in embryos to metabolism in larvae, and increased lipid metabolism in adults. We expect that this transcriptome will provide a useful resource for future analyses to better understand the molecular basis of the evolution of eusociality and, more generally, phenotypic plasticity. Genetics Society of America 2015-08-14 /pmc/articles/PMC4592995/ /pubmed/26276382 http://dx.doi.org/10.1534/g3.115.021261 Text en Copyright © 2015 Jones et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigations Jones, Beryl M. Wcislo, William T. Robinson, Gene E. Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title | Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title_full | Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title_fullStr | Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title_full_unstemmed | Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title_short | Developmental Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis |
title_sort | developmental transcriptome for a facultatively eusocial bee, megalopta genalis |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592995/ https://www.ncbi.nlm.nih.gov/pubmed/26276382 http://dx.doi.org/10.1534/g3.115.021261 |
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