Cargando…

Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park

Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S rRNA sequences confirmed the classification of the strain as a G. thermoglucos...

Descripción completa

Detalles Bibliográficos
Autores principales: Brumm, Phillip J., Land, Miriam L., Mead, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593210/
https://www.ncbi.nlm.nih.gov/pubmed/26442136
http://dx.doi.org/10.1186/s40793-015-0031-z
_version_ 1782393295137144832
author Brumm, Phillip J.
Land, Miriam L.
Mead, David A.
author_facet Brumm, Phillip J.
Land, Miriam L.
Mead, David A.
author_sort Brumm, Phillip J.
collection PubMed
description Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S rRNA sequences confirmed the classification of the strain as a G. thermoglucosidasius species. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). The genome of G. thermoglucosidasius C56-YS93 consists of one circular chromosome of 3,893,306 bp and two circular plasmids of 80,849 and 19,638 bp and an average G + C content of 43.93 %. G. thermoglucosidasius C56-YS93 possesses a xylan degradation cluster not found in the other G. thermoglucosidasius sequenced strains. This cluster appears to be related to the xylan degradation cluster found in G. stearothermophilus. G. thermoglucosidasius C56-YS93 possesses two plasmids not found in the other two strains. One plasmid contains a novel gene cluster coding for proteins involved in proline degradation and metabolism, the other contains a collection of mostly hypothetical proteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0031-z) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4593210
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45932102015-10-06 Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park Brumm, Phillip J. Land, Miriam L. Mead, David A. Stand Genomic Sci Short Genome Report Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S rRNA sequences confirmed the classification of the strain as a G. thermoglucosidasius species. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). The genome of G. thermoglucosidasius C56-YS93 consists of one circular chromosome of 3,893,306 bp and two circular plasmids of 80,849 and 19,638 bp and an average G + C content of 43.93 %. G. thermoglucosidasius C56-YS93 possesses a xylan degradation cluster not found in the other G. thermoglucosidasius sequenced strains. This cluster appears to be related to the xylan degradation cluster found in G. stearothermophilus. G. thermoglucosidasius C56-YS93 possesses two plasmids not found in the other two strains. One plasmid contains a novel gene cluster coding for proteins involved in proline degradation and metabolism, the other contains a collection of mostly hypothetical proteins. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0031-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-05 /pmc/articles/PMC4593210/ /pubmed/26442136 http://dx.doi.org/10.1186/s40793-015-0031-z Text en © Brumm et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0 (http://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Genome Report
Brumm, Phillip J.
Land, Miriam L.
Mead, David A.
Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title_full Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title_fullStr Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title_full_unstemmed Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title_short Complete genome sequence of Geobacillus thermoglucosidasius C56-YS93, a novel biomass degrader isolated from obsidian hot spring in Yellowstone National Park
title_sort complete genome sequence of geobacillus thermoglucosidasius c56-ys93, a novel biomass degrader isolated from obsidian hot spring in yellowstone national park
topic Short Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593210/
https://www.ncbi.nlm.nih.gov/pubmed/26442136
http://dx.doi.org/10.1186/s40793-015-0031-z
work_keys_str_mv AT brummphillipj completegenomesequenceofgeobacillusthermoglucosidasiusc56ys93anovelbiomassdegraderisolatedfromobsidianhotspringinyellowstonenationalpark
AT landmiriaml completegenomesequenceofgeobacillusthermoglucosidasiusc56ys93anovelbiomassdegraderisolatedfromobsidianhotspringinyellowstonenationalpark
AT meaddavida completegenomesequenceofgeobacillusthermoglucosidasiusc56ys93anovelbiomassdegraderisolatedfromobsidianhotspringinyellowstonenationalpark