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Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection

Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, nonin...

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Autores principales: Min, Kaiyin, Ji, Bin, Zhao, Min, Ji, Tiefeng, Chen, Bin, Fang, Xuedong, Ma, Qingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593522/
https://www.ncbi.nlm.nih.gov/pubmed/26437463
http://dx.doi.org/10.1371/journal.pone.0139471
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author Min, Kaiyin
Ji, Bin
Zhao, Min
Ji, Tiefeng
Chen, Bin
Fang, Xuedong
Ma, Qingjie
author_facet Min, Kaiyin
Ji, Bin
Zhao, Min
Ji, Tiefeng
Chen, Bin
Fang, Xuedong
Ma, Qingjie
author_sort Min, Kaiyin
collection PubMed
description Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, noninvasive visualization and quantification of MMP in vivo are of great interest in basic research and clinical application for breast cancer early diagnosis. Herein, we developed a (99m)Tc labeled membrane type I matrix metalloproteinase (MT1-MMP) specific binding peptide, [(99m)Tc]-(HYNIC-AF7p)(tricine)(TPPTS), for in vivo detection of MDA-MB-231 breast tumor by single photon emission computed tomography (SPECT). [(99m)Tc]-(HYNIC-AF7p)(tricine)(TPPTS) demonstrated nice biostability and high MT1-MMP binding affinity in vitro and in vivo. Tumor-to-muscle ratio was found to reach to the highest (4.17±0.49) at 2 hour after intravenously administration of [(99m)Tc]-(HYNIC-AF7P)(tricine)(TPPTS) into MDA-MB-231 tumor bearing mice. Overall, [(99m)Tc]-(HYNIC-AF7P)(tricine)(TPPTS) demonstrated great potential for MT1-MMP targeted detection in vivo and it would be a promising molecular imaging probe that are probably beneficial to breast cancer early diagnoses.
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spelling pubmed-45935222015-10-14 Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection Min, Kaiyin Ji, Bin Zhao, Min Ji, Tiefeng Chen, Bin Fang, Xuedong Ma, Qingjie PLoS One Research Article Breast cancer is one of the most frequent and aggressive primary tumors among women of all races. Matrix metalloproteinase (MMPs), a family of zinc- and calcium-dependent secreted or membrane anchored endopeptidases, is overexpressed in varieties of diseases including breast cancer. Therefore, noninvasive visualization and quantification of MMP in vivo are of great interest in basic research and clinical application for breast cancer early diagnosis. Herein, we developed a (99m)Tc labeled membrane type I matrix metalloproteinase (MT1-MMP) specific binding peptide, [(99m)Tc]-(HYNIC-AF7p)(tricine)(TPPTS), for in vivo detection of MDA-MB-231 breast tumor by single photon emission computed tomography (SPECT). [(99m)Tc]-(HYNIC-AF7p)(tricine)(TPPTS) demonstrated nice biostability and high MT1-MMP binding affinity in vitro and in vivo. Tumor-to-muscle ratio was found to reach to the highest (4.17±0.49) at 2 hour after intravenously administration of [(99m)Tc]-(HYNIC-AF7P)(tricine)(TPPTS) into MDA-MB-231 tumor bearing mice. Overall, [(99m)Tc]-(HYNIC-AF7P)(tricine)(TPPTS) demonstrated great potential for MT1-MMP targeted detection in vivo and it would be a promising molecular imaging probe that are probably beneficial to breast cancer early diagnoses. Public Library of Science 2015-10-05 /pmc/articles/PMC4593522/ /pubmed/26437463 http://dx.doi.org/10.1371/journal.pone.0139471 Text en © 2015 Min et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Min, Kaiyin
Ji, Bin
Zhao, Min
Ji, Tiefeng
Chen, Bin
Fang, Xuedong
Ma, Qingjie
Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title_full Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title_fullStr Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title_full_unstemmed Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title_short Development of a Radiolabeled Peptide-Based Probe Targeting MT1-MMP for Breast Cancer Detection
title_sort development of a radiolabeled peptide-based probe targeting mt1-mmp for breast cancer detection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593522/
https://www.ncbi.nlm.nih.gov/pubmed/26437463
http://dx.doi.org/10.1371/journal.pone.0139471
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