Optimizing Production of Antigens and Fabs in the Context of Generating Recombinant Antibodies to Human Proteins

We developed and optimized a high-throughput project workflow to generate renewable recombinant antibodies to human proteins involved in epigenetic signalling. Three different strategies to produce phage display compatible protein antigens in bacterial systems were compared, and we found that in viv...

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Detalles Bibliográficos
Autores principales: Zhong, Nan, Loppnau, Peter, Seitova, Alma, Ravichandran, Mani, Fenner, Maria, Jain, Harshika, Bhattacharya, Anandi, Hutchinson, Ashley, Paduch, Marcin, Lu, Vincent, Olszewski, Michal, Kossiakoff, Anthony A., Dowdell, Evan, Koide, Akiko, Koide, Shohei, Huang, Haiming, Nadeem, Vincent, Sidhu, Sachdev S., Greenblatt, Jack F., Marcon, Edyta, Arrowsmith, Cheryl H., Edwards, Aled M., Gräslund, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593582/
https://www.ncbi.nlm.nih.gov/pubmed/26437229
http://dx.doi.org/10.1371/journal.pone.0139695

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