Synthesis of double-clickable functionalised graphene oxide for biological applications

Azide- and alkyne-double functionalised graphene oxide (Click(2) GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was foun...

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Detalles Bibliográficos
Autores principales: Mei, Kuo-Ching, Rubio, Noelia, Costa, Pedro M., Kafa, Houmam, Abbate, Vincenzo, Festy, Frederic, Bansal, Sukhvinder S., Hider, Robert C., Al-Jamal, Khuloud T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594119/
https://www.ncbi.nlm.nih.gov/pubmed/26295072
http://dx.doi.org/10.1039/c5cc05412e
Descripción
Sumario:Azide- and alkyne-double functionalised graphene oxide (Click(2) GO) was synthesised and characterised with attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA) and Raman spectroscopy. Fourteen-percentage increase in azide content was found, after pre-treatment of GO with meta-chloroperoxybenzoic acid (mCPBA), determined with elemental analysis. No effect on A549 cell viability was found, up to 100 μg mL(–1) and 72 h of incubation, determined with the modified lactate dehydrogenase (mLDH) assay. Two sequential copper(i) catalysed azide–alkyne cycloaddition (CuAAC) reactions were performed to conjugate the propargyl-modified blood–brain barrier targeting peptide Angiopep-2, and a bis-azide polyethylene glycol (M (W) = 3500), to the Click(2) GO. The final conjugate was characterised with ATR-FTIR and TGA.

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