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Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus

Genetic linkage maps are critical and indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density and high-resolution linkage maps has become achievable in marine organisms lacking sufficient...

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Autores principales: Tian, Meilin, Li, Yangping, Jing, Jing, Mu, Chuang, Du, Huixia, Dou, Jinzhuang, Mao, Junxia, Li, Xue, Jiao, Wenqian, Wang, Yangfan, Hu, Xiaoli, Wang, Shi, Wang, Ruijia, Bao, Zhenmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594301/
https://www.ncbi.nlm.nih.gov/pubmed/26439740
http://dx.doi.org/10.1038/srep14852
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author Tian, Meilin
Li, Yangping
Jing, Jing
Mu, Chuang
Du, Huixia
Dou, Jinzhuang
Mao, Junxia
Li, Xue
Jiao, Wenqian
Wang, Yangfan
Hu, Xiaoli
Wang, Shi
Wang, Ruijia
Bao, Zhenmin
author_facet Tian, Meilin
Li, Yangping
Jing, Jing
Mu, Chuang
Du, Huixia
Dou, Jinzhuang
Mao, Junxia
Li, Xue
Jiao, Wenqian
Wang, Yangfan
Hu, Xiaoli
Wang, Shi
Wang, Ruijia
Bao, Zhenmin
author_sort Tian, Meilin
collection PubMed
description Genetic linkage maps are critical and indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density and high-resolution linkage maps has become achievable in marine organisms lacking sufficient genomic resources, such as echinoderms. In this study, high-density, high-resolution genetic map was constructed for a sea cucumber species, Apostichopus japonicus, utilizing the 2b-restriction site-associated DNA (2b-RAD) method. A total of 7839 markers were anchored to the linkage map with the map coverage of 99.57%, to our knowledge, this is the highest marker density among echinoderm species. QTL mapping and association analysis consistently captured one growth-related QTL located in a 5 cM region of linkage group (LG) 5. An annotated candidate gene, retinoblastoma-binding protein 5 (RbBP5), which has been reported to be an important regulator of cell proliferation, was recognized in the QTL region. This linkage map represents a powerful tool for research involving both fine-scale QTL mapping and marker assisted selection (MAS), and will facilitate chromosome assignment and improve the whole-genome assembly of sea cucumber in the future.
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spelling pubmed-45943012015-10-13 Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus Tian, Meilin Li, Yangping Jing, Jing Mu, Chuang Du, Huixia Dou, Jinzhuang Mao, Junxia Li, Xue Jiao, Wenqian Wang, Yangfan Hu, Xiaoli Wang, Shi Wang, Ruijia Bao, Zhenmin Sci Rep Article Genetic linkage maps are critical and indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density and high-resolution linkage maps has become achievable in marine organisms lacking sufficient genomic resources, such as echinoderms. In this study, high-density, high-resolution genetic map was constructed for a sea cucumber species, Apostichopus japonicus, utilizing the 2b-restriction site-associated DNA (2b-RAD) method. A total of 7839 markers were anchored to the linkage map with the map coverage of 99.57%, to our knowledge, this is the highest marker density among echinoderm species. QTL mapping and association analysis consistently captured one growth-related QTL located in a 5 cM region of linkage group (LG) 5. An annotated candidate gene, retinoblastoma-binding protein 5 (RbBP5), which has been reported to be an important regulator of cell proliferation, was recognized in the QTL region. This linkage map represents a powerful tool for research involving both fine-scale QTL mapping and marker assisted selection (MAS), and will facilitate chromosome assignment and improve the whole-genome assembly of sea cucumber in the future. Nature Publishing Group 2015-10-06 /pmc/articles/PMC4594301/ /pubmed/26439740 http://dx.doi.org/10.1038/srep14852 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tian, Meilin
Li, Yangping
Jing, Jing
Mu, Chuang
Du, Huixia
Dou, Jinzhuang
Mao, Junxia
Li, Xue
Jiao, Wenqian
Wang, Yangfan
Hu, Xiaoli
Wang, Shi
Wang, Ruijia
Bao, Zhenmin
Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title_full Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title_fullStr Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title_full_unstemmed Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title_short Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Sea Cucumber Apostichopus japonicus
title_sort construction of a high-density genetic map and quantitative trait locus mapping in the sea cucumber apostichopus japonicus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594301/
https://www.ncbi.nlm.nih.gov/pubmed/26439740
http://dx.doi.org/10.1038/srep14852
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