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Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa

The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses,...

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Autores principales: Dornas, Fábio P., Khalil, Jacques Y. B., Pagnier, Isabelle, Raoult, Didier, Abrahão, Jônatas, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594340/
https://www.ncbi.nlm.nih.gov/pubmed/26500630
http://dx.doi.org/10.3389/fmicb.2015.01086
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author Dornas, Fábio P.
Khalil, Jacques Y. B.
Pagnier, Isabelle
Raoult, Didier
Abrahão, Jônatas
La Scola, Bernard
author_facet Dornas, Fábio P.
Khalil, Jacques Y. B.
Pagnier, Isabelle
Raoult, Didier
Abrahão, Jônatas
La Scola, Bernard
author_sort Dornas, Fábio P.
collection PubMed
description The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses, we propose the use of different species of amoeba as a cellular support. The aim of this work was to isolate new Brazilian giant viruses by comparing the protozoa Acanthamoeba castellanii, A. polyphaga, A. griffini, and Vermamoeba vermiformis (VV) as a platform for cellular isolation using environmental samples. One hundred samples were collected from 3 different areas in September 2014 in the Pampulha lagoon of Belo Horizonte city, Minas Gerais, Brazil. PCR was used to identify the isolated viruses, along with hemacolor staining, labelling fluorescence and electron microscopy. A total of 69 viruses were isolated. The highest ratio of isolation was found in A. polyphaga (46.38%) and the lowest in VV (0%). Mimiviruses were the most frequently isolated. One Marseillevirus and one Pandoravirus were also isolated. With Brazilian environmental samples, we demonstrated the high rate of lineage A mimiviruses. This work demonstrates how these viruses survive and circulate in nature as well the differences between protozoa as a platform for cellular isolation.
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spelling pubmed-45943402015-10-23 Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa Dornas, Fábio P. Khalil, Jacques Y. B. Pagnier, Isabelle Raoult, Didier Abrahão, Jônatas La Scola, Bernard Front Microbiol Microbiology The Megavirales are a newly described order capable of infecting different types of eukaryotic hosts. For the most part, the natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular methods and co-cultures. To isolate giant viruses, we propose the use of different species of amoeba as a cellular support. The aim of this work was to isolate new Brazilian giant viruses by comparing the protozoa Acanthamoeba castellanii, A. polyphaga, A. griffini, and Vermamoeba vermiformis (VV) as a platform for cellular isolation using environmental samples. One hundred samples were collected from 3 different areas in September 2014 in the Pampulha lagoon of Belo Horizonte city, Minas Gerais, Brazil. PCR was used to identify the isolated viruses, along with hemacolor staining, labelling fluorescence and electron microscopy. A total of 69 viruses were isolated. The highest ratio of isolation was found in A. polyphaga (46.38%) and the lowest in VV (0%). Mimiviruses were the most frequently isolated. One Marseillevirus and one Pandoravirus were also isolated. With Brazilian environmental samples, we demonstrated the high rate of lineage A mimiviruses. This work demonstrates how these viruses survive and circulate in nature as well the differences between protozoa as a platform for cellular isolation. Frontiers Media S.A. 2015-10-06 /pmc/articles/PMC4594340/ /pubmed/26500630 http://dx.doi.org/10.3389/fmicb.2015.01086 Text en Copyright © 2015 Dornas, Khalil, Pagnier, Raoult, Abrahão and La Scola. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dornas, Fábio P.
Khalil, Jacques Y. B.
Pagnier, Isabelle
Raoult, Didier
Abrahão, Jônatas
La Scola, Bernard
Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title_full Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title_fullStr Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title_full_unstemmed Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title_short Isolation of new Brazilian giant viruses from environmental samples using a panel of protozoa
title_sort isolation of new brazilian giant viruses from environmental samples using a panel of protozoa
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594340/
https://www.ncbi.nlm.nih.gov/pubmed/26500630
http://dx.doi.org/10.3389/fmicb.2015.01086
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