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Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium
Succinate is a building block compound that the U.S. Department of Energy (DOE) has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular cyanobacterium Synechocystis sp. PCC 6803. S...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594341/ https://www.ncbi.nlm.nih.gov/pubmed/26500619 http://dx.doi.org/10.3389/fmicb.2015.01064 |
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author | Osanai, Takashi Shirai, Tomokazu Iijima, Hiroko Nakaya, Yuka Okamoto, Mami Kondo, Akihiko Hirai, Masami Y. |
author_facet | Osanai, Takashi Shirai, Tomokazu Iijima, Hiroko Nakaya, Yuka Okamoto, Mami Kondo, Akihiko Hirai, Masami Y. |
author_sort | Osanai, Takashi |
collection | PubMed |
description | Succinate is a building block compound that the U.S. Department of Energy (DOE) has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular cyanobacterium Synechocystis sp. PCC 6803. Succinate was excreted under dark, anaerobic conditions, and its production level increased by knocking out ackA, which encodes an acetate kinase, and by overexpressing sigE, which encodes an RNA polymerase sigma factor. Glycogen catabolism and organic acid biosynthesis were enhanced in the mutant lacking ackA and overexpressing sigE, leading to an increase in succinate production reaching five times of the wild-type levels. Our genetic and metabolomic analyses thus demonstrated the effect of genetic manipulation of a metabolic enzyme and a transcriptional regulator on succinate excretion from this cyanobacterium with the data based on metabolomic technique. |
format | Online Article Text |
id | pubmed-4594341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45943412015-10-23 Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium Osanai, Takashi Shirai, Tomokazu Iijima, Hiroko Nakaya, Yuka Okamoto, Mami Kondo, Akihiko Hirai, Masami Y. Front Microbiol Microbiology Succinate is a building block compound that the U.S. Department of Energy (DOE) has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular cyanobacterium Synechocystis sp. PCC 6803. Succinate was excreted under dark, anaerobic conditions, and its production level increased by knocking out ackA, which encodes an acetate kinase, and by overexpressing sigE, which encodes an RNA polymerase sigma factor. Glycogen catabolism and organic acid biosynthesis were enhanced in the mutant lacking ackA and overexpressing sigE, leading to an increase in succinate production reaching five times of the wild-type levels. Our genetic and metabolomic analyses thus demonstrated the effect of genetic manipulation of a metabolic enzyme and a transcriptional regulator on succinate excretion from this cyanobacterium with the data based on metabolomic technique. Frontiers Media S.A. 2015-10-06 /pmc/articles/PMC4594341/ /pubmed/26500619 http://dx.doi.org/10.3389/fmicb.2015.01064 Text en Copyright © 2015 Osanai, Shirai, Iijima, Nakaya, Okamoto, Kondo and Hirai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Osanai, Takashi Shirai, Tomokazu Iijima, Hiroko Nakaya, Yuka Okamoto, Mami Kondo, Akihiko Hirai, Masami Y. Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title | Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title_full | Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title_fullStr | Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title_full_unstemmed | Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title_short | Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
title_sort | genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594341/ https://www.ncbi.nlm.nih.gov/pubmed/26500619 http://dx.doi.org/10.3389/fmicb.2015.01064 |
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