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Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphos...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taylor & Francis
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594416/ https://www.ncbi.nlm.nih.gov/pubmed/26480268 http://dx.doi.org/10.1080/19420889.2015.1017174 |
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author | Huang, Guozhong Docampo, Roberto |
author_facet | Huang, Guozhong Docampo, Roberto |
author_sort | Huang, Guozhong |
collection | PubMed |
description | Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphosphate (polyP) content. Our proteomic analysis of acidocalcisomes of T. brucei procyclic stages, together with in situ epitope-tagging and immunofluorescence assays with specific antibodies against selected proteins, established the presence of 2 H(+) pumps, a vacuolar H(+)-ATPase and a vacuolar H(+)-pyrophosphatase, that acidify the organelles as well as of a number of transporters and channels involved in phosphate metabolism, cation uptake and calcium signaling. Together with recent work in other organisms, these results provide direct evidence that acidocalcisomes are especially adapted to accumulate polyP bound to cations and for calcium signaling. |
format | Online Article Text |
id | pubmed-4594416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-45944162015-10-16 Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling Huang, Guozhong Docampo, Roberto Commun Integr Biol Article Addendum Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphosphate (polyP) content. Our proteomic analysis of acidocalcisomes of T. brucei procyclic stages, together with in situ epitope-tagging and immunofluorescence assays with specific antibodies against selected proteins, established the presence of 2 H(+) pumps, a vacuolar H(+)-ATPase and a vacuolar H(+)-pyrophosphatase, that acidify the organelles as well as of a number of transporters and channels involved in phosphate metabolism, cation uptake and calcium signaling. Together with recent work in other organisms, these results provide direct evidence that acidocalcisomes are especially adapted to accumulate polyP bound to cations and for calcium signaling. Taylor & Francis 2015-05-06 /pmc/articles/PMC4594416/ /pubmed/26480268 http://dx.doi.org/10.1080/19420889.2015.1017174 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Article Addendum Huang, Guozhong Docampo, Roberto Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title | Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title_full | Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title_fullStr | Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title_full_unstemmed | Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title_short | Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
title_sort | proteomic analysis of acidocalcisomes of trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling |
topic | Article Addendum |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594416/ https://www.ncbi.nlm.nih.gov/pubmed/26480268 http://dx.doi.org/10.1080/19420889.2015.1017174 |
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