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Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling

Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphos...

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Autores principales: Huang, Guozhong, Docampo, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594416/
https://www.ncbi.nlm.nih.gov/pubmed/26480268
http://dx.doi.org/10.1080/19420889.2015.1017174
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author Huang, Guozhong
Docampo, Roberto
author_facet Huang, Guozhong
Docampo, Roberto
author_sort Huang, Guozhong
collection PubMed
description Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphosphate (polyP) content. Our proteomic analysis of acidocalcisomes of T. brucei procyclic stages, together with in situ epitope-tagging and immunofluorescence assays with specific antibodies against selected proteins, established the presence of 2 H(+) pumps, a vacuolar H(+)-ATPase and a vacuolar H(+)-pyrophosphatase, that acidify the organelles as well as of a number of transporters and channels involved in phosphate metabolism, cation uptake and calcium signaling. Together with recent work in other organisms, these results provide direct evidence that acidocalcisomes are especially adapted to accumulate polyP bound to cations and for calcium signaling.
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spelling pubmed-45944162015-10-16 Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling Huang, Guozhong Docampo, Roberto Commun Integr Biol Article Addendum Trypanosoma brucei, the causative agent of African trypanosomiasis, is a unicellular parasite that possesses lysosome-related organelles known as acidocalcisomes. These organelles have been found from bacteria to human cells, and are characterized by their acidic nature and high calcium and polyphosphate (polyP) content. Our proteomic analysis of acidocalcisomes of T. brucei procyclic stages, together with in situ epitope-tagging and immunofluorescence assays with specific antibodies against selected proteins, established the presence of 2 H(+) pumps, a vacuolar H(+)-ATPase and a vacuolar H(+)-pyrophosphatase, that acidify the organelles as well as of a number of transporters and channels involved in phosphate metabolism, cation uptake and calcium signaling. Together with recent work in other organisms, these results provide direct evidence that acidocalcisomes are especially adapted to accumulate polyP bound to cations and for calcium signaling. Taylor & Francis 2015-05-06 /pmc/articles/PMC4594416/ /pubmed/26480268 http://dx.doi.org/10.1080/19420889.2015.1017174 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Article Addendum
Huang, Guozhong
Docampo, Roberto
Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title_full Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title_fullStr Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title_full_unstemmed Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title_short Proteomic analysis of acidocalcisomes of Trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
title_sort proteomic analysis of acidocalcisomes of trypanosoma brucei uncovers their role in phosphate metabolism, cation homeostasis, and calcium signaling
topic Article Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594416/
https://www.ncbi.nlm.nih.gov/pubmed/26480268
http://dx.doi.org/10.1080/19420889.2015.1017174
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