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Electrical behaviour of dendritic spines as revealed by voltage imaging
Thousands of dendritic spines on individual neurons process information and mediate plasticity by generating electrical input signals using a sophisticated assembly of transmitter receptors and voltage-sensitive ion channel molecules. Our understanding, however, of the electrical behaviour of spines...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594633/ https://www.ncbi.nlm.nih.gov/pubmed/26436431 http://dx.doi.org/10.1038/ncomms9436 |
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author | Popovic, Marko A. Carnevale, Nicholas Rozsa, Balazs Zecevic, Dejan |
author_facet | Popovic, Marko A. Carnevale, Nicholas Rozsa, Balazs Zecevic, Dejan |
author_sort | Popovic, Marko A. |
collection | PubMed |
description | Thousands of dendritic spines on individual neurons process information and mediate plasticity by generating electrical input signals using a sophisticated assembly of transmitter receptors and voltage-sensitive ion channel molecules. Our understanding, however, of the electrical behaviour of spines is limited because it has not been possible to record input signals from these structures with adequate sensitivity and spatiotemporal resolution. Current interpretation of indirect data and speculations based on theoretical considerations are inconclusive. Here we use an electrochromic voltage-sensitive dye which acts as a transmembrane optical voltmeter with a linear scale to directly monitor electrical signals from individual spines on thin basal dendrites. The results show that synapses on these spines are not electrically isolated by the spine neck to a significant extent. Electrically, they behave as if they are located directly on dendrites. |
format | Online Article Text |
id | pubmed-4594633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45946332015-10-21 Electrical behaviour of dendritic spines as revealed by voltage imaging Popovic, Marko A. Carnevale, Nicholas Rozsa, Balazs Zecevic, Dejan Nat Commun Article Thousands of dendritic spines on individual neurons process information and mediate plasticity by generating electrical input signals using a sophisticated assembly of transmitter receptors and voltage-sensitive ion channel molecules. Our understanding, however, of the electrical behaviour of spines is limited because it has not been possible to record input signals from these structures with adequate sensitivity and spatiotemporal resolution. Current interpretation of indirect data and speculations based on theoretical considerations are inconclusive. Here we use an electrochromic voltage-sensitive dye which acts as a transmembrane optical voltmeter with a linear scale to directly monitor electrical signals from individual spines on thin basal dendrites. The results show that synapses on these spines are not electrically isolated by the spine neck to a significant extent. Electrically, they behave as if they are located directly on dendrites. Nature Pub. Group 2015-10-05 /pmc/articles/PMC4594633/ /pubmed/26436431 http://dx.doi.org/10.1038/ncomms9436 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Popovic, Marko A. Carnevale, Nicholas Rozsa, Balazs Zecevic, Dejan Electrical behaviour of dendritic spines as revealed by voltage imaging |
title | Electrical behaviour of dendritic spines as revealed by voltage imaging |
title_full | Electrical behaviour of dendritic spines as revealed by voltage imaging |
title_fullStr | Electrical behaviour of dendritic spines as revealed by voltage imaging |
title_full_unstemmed | Electrical behaviour of dendritic spines as revealed by voltage imaging |
title_short | Electrical behaviour of dendritic spines as revealed by voltage imaging |
title_sort | electrical behaviour of dendritic spines as revealed by voltage imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594633/ https://www.ncbi.nlm.nih.gov/pubmed/26436431 http://dx.doi.org/10.1038/ncomms9436 |
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