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Role of augmented transferrin during the retraining for undeveloped left ventricle
Transposition of great arteries (TGA) is a common congenital heart disease. Left ventricle (LV) is rapidly regressing and pulmonary artery banding (PAB) is utilized to retrain the undeveloped LV. Hence, it offered a unique human disease model to investigate the process of LV hypertrophy under pressu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594683/ https://www.ncbi.nlm.nih.gov/pubmed/26099594 http://dx.doi.org/10.1111/jcmm.12627 |
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author | Wei, Wei Wu, Yihe Ying, Yongquan Li, Shoujun Hu, Shengshou Zhang, Hao |
author_facet | Wei, Wei Wu, Yihe Ying, Yongquan Li, Shoujun Hu, Shengshou Zhang, Hao |
author_sort | Wei, Wei |
collection | PubMed |
description | Transposition of great arteries (TGA) is a common congenital heart disease. Left ventricle (LV) is rapidly regressing and pulmonary artery banding (PAB) is utilized to retrain the undeveloped LV. Hence, it offered a unique human disease model to investigate the process of LV hypertrophy under pressure overload. Eight late referred children with TGA were enrolled. The plasma was collected at the 30 min. before and 48 hrs after PAB, and 25 proteins were identified as having significant change in proteomic analysis. Transferrin (TF) and ceruloplasmin were then confirmed. After 48 hrs incubation with TF, the size of human induced pluripotent stem cell-derived cardiomyocytes increased by two times as large as control. Meanwhile, protein synthesis and the expression of natriuretic peptide precursor A and B were significantly enhanced. TF treatment also activated both extracellular signal-regulated kinase 1/2 and activated protein kinase singling pathways. Our data provided a link to molecular components and pathways that might be involved in LV retraining. TF severed as the carrier to delivery irons, and could directly stimulate cardiomyocytes hypertrophy. TF administration may hold therapeutic potential for the biological LV retraining. |
format | Online Article Text |
id | pubmed-4594683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley & Sons, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45946832015-10-09 Role of augmented transferrin during the retraining for undeveloped left ventricle Wei, Wei Wu, Yihe Ying, Yongquan Li, Shoujun Hu, Shengshou Zhang, Hao J Cell Mol Med Original Articles Transposition of great arteries (TGA) is a common congenital heart disease. Left ventricle (LV) is rapidly regressing and pulmonary artery banding (PAB) is utilized to retrain the undeveloped LV. Hence, it offered a unique human disease model to investigate the process of LV hypertrophy under pressure overload. Eight late referred children with TGA were enrolled. The plasma was collected at the 30 min. before and 48 hrs after PAB, and 25 proteins were identified as having significant change in proteomic analysis. Transferrin (TF) and ceruloplasmin were then confirmed. After 48 hrs incubation with TF, the size of human induced pluripotent stem cell-derived cardiomyocytes increased by two times as large as control. Meanwhile, protein synthesis and the expression of natriuretic peptide precursor A and B were significantly enhanced. TF treatment also activated both extracellular signal-regulated kinase 1/2 and activated protein kinase singling pathways. Our data provided a link to molecular components and pathways that might be involved in LV retraining. TF severed as the carrier to delivery irons, and could directly stimulate cardiomyocytes hypertrophy. TF administration may hold therapeutic potential for the biological LV retraining. John Wiley & Sons, Ltd 2015-10 2015-06-23 /pmc/articles/PMC4594683/ /pubmed/26099594 http://dx.doi.org/10.1111/jcmm.12627 Text en © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Wei, Wei Wu, Yihe Ying, Yongquan Li, Shoujun Hu, Shengshou Zhang, Hao Role of augmented transferrin during the retraining for undeveloped left ventricle |
title | Role of augmented transferrin during the retraining for undeveloped left ventricle |
title_full | Role of augmented transferrin during the retraining for undeveloped left ventricle |
title_fullStr | Role of augmented transferrin during the retraining for undeveloped left ventricle |
title_full_unstemmed | Role of augmented transferrin during the retraining for undeveloped left ventricle |
title_short | Role of augmented transferrin during the retraining for undeveloped left ventricle |
title_sort | role of augmented transferrin during the retraining for undeveloped left ventricle |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594683/ https://www.ncbi.nlm.nih.gov/pubmed/26099594 http://dx.doi.org/10.1111/jcmm.12627 |
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