A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

BACKGROUND: Recombinant protein production using Escherichia coli as expression host is highly efficient, however, it also induces strong host cell metabolic burden. Energy and biomass precursors are withdrawn from the host’s metabolism as they are required for plasmid replication, heterologous gene...

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Detalles Bibliográficos
Autores principales: Rahmen, Natalie, Schlupp, Christian D., Mitsunaga, Hitoshi, Fulton, Alexander, Aryani, Tita, Esch, Lara, Schaffrath, Ulrich, Fukuzaki, Eiichiro, Jaeger, Karl-Erich, Büchs, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595056/
https://www.ncbi.nlm.nih.gov/pubmed/26438243
http://dx.doi.org/10.1186/s12934-015-0348-8

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