Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

The generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and d...

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Detalles Bibliográficos
Autores principales: Xu, Fen, Liu, Junli, Deng, Jie, Chen, Xiaolei, Wang, Yuan, Xu, Pengchao, Cheng, Lin, Fu, Yanli, Cheng, Fuyi, Yao, Yunqi, Zhang, Yujing, Huang, Meijuan, Yu, Dechao, Wei, Yuquan, Deng, Hongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595267/
https://www.ncbi.nlm.nih.gov/pubmed/26438426
http://dx.doi.org/10.1186/s13287-015-0181-3
Descripción
Sumario:The generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity. Upon transplantation into mice with carbon tetrachloride (CCl(4))-induced acute fulminant liver failure, iHeps restore the liver function and prolong survival. The work could contribute to the development of alternative strategies to obtain nonhepatic cell-derived mature hepatocytes with potential for biomedical and pharmaceutical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-015-0181-3) contains supplementary material, which is available to authorized users.

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