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Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595308/ https://www.ncbi.nlm.nih.gov/pubmed/26438125 http://dx.doi.org/10.1186/s12934-015-0339-9 |
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author | Liu, Xuemei Zuo, Mingxing Wang, Ting Sun, Yunjun Liu, Shuang Hu, Shengbiao He, Hao Yang, Qi Rang, Jie Quan, Meifang Xia, Liqiu Ding, Xuezhi |
author_facet | Liu, Xuemei Zuo, Mingxing Wang, Ting Sun, Yunjun Liu, Shuang Hu, Shengbiao He, Hao Yang, Qi Rang, Jie Quan, Meifang Xia, Liqiu Ding, Xuezhi |
author_sort | Liu, Xuemei |
collection | PubMed |
description | BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported. RESULTS: The ICP are mainly composed of Cry1Ca, Cry1Ac, and Cry1Da, which have molecular weights of about 130 kDa. ICP production was most efficient when Cu(2+) was added at concentrations ranging from 10(−6) to 10(−4) mol/L at an initial pH of 8.0. Addition of Cu(2+) also evidently increased the toxicity of fermentation broth to Spodoptera exigua and Helicoverpa armigera. After analyzing changes in proteome and fermentation parameters caused by Cu(2+) addition, we propose that Cu(2+) increases PhaR expression and consequently changes the carbon flow. More carbon sources was used to produce intracellular poly-β-hydroxybutyrate (PHB). Increases in PHB as a storage material bring about increases of ICP production. CONCLUSIONS: Bacillus thuringiensis X022 mainly expresses Cry1Ca, Cry1Ac, and Cry1Da. Cu(2+) increases the expression of Cry1Da, Cry1Ca, and also enhances the toxicity of fermentation broth to S. exigua and H. armigera. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0339-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4595308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45953082015-10-08 Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 Liu, Xuemei Zuo, Mingxing Wang, Ting Sun, Yunjun Liu, Shuang Hu, Shengbiao He, Hao Yang, Qi Rang, Jie Quan, Meifang Xia, Liqiu Ding, Xuezhi Microb Cell Fact Research BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported. RESULTS: The ICP are mainly composed of Cry1Ca, Cry1Ac, and Cry1Da, which have molecular weights of about 130 kDa. ICP production was most efficient when Cu(2+) was added at concentrations ranging from 10(−6) to 10(−4) mol/L at an initial pH of 8.0. Addition of Cu(2+) also evidently increased the toxicity of fermentation broth to Spodoptera exigua and Helicoverpa armigera. After analyzing changes in proteome and fermentation parameters caused by Cu(2+) addition, we propose that Cu(2+) increases PhaR expression and consequently changes the carbon flow. More carbon sources was used to produce intracellular poly-β-hydroxybutyrate (PHB). Increases in PHB as a storage material bring about increases of ICP production. CONCLUSIONS: Bacillus thuringiensis X022 mainly expresses Cry1Ca, Cry1Ac, and Cry1Da. Cu(2+) increases the expression of Cry1Da, Cry1Ca, and also enhances the toxicity of fermentation broth to S. exigua and H. armigera. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0339-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-05 /pmc/articles/PMC4595308/ /pubmed/26438125 http://dx.doi.org/10.1186/s12934-015-0339-9 Text en © Liu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Liu, Xuemei Zuo, Mingxing Wang, Ting Sun, Yunjun Liu, Shuang Hu, Shengbiao He, Hao Yang, Qi Rang, Jie Quan, Meifang Xia, Liqiu Ding, Xuezhi Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title | Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title_full | Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title_fullStr | Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title_full_unstemmed | Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title_short | Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 |
title_sort | proteomic analysis of the influence of cu(2+) on the crystal protein production of bacillus thuringiensis x022 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595308/ https://www.ncbi.nlm.nih.gov/pubmed/26438125 http://dx.doi.org/10.1186/s12934-015-0339-9 |
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