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Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022

BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However...

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Autores principales: Liu, Xuemei, Zuo, Mingxing, Wang, Ting, Sun, Yunjun, Liu, Shuang, Hu, Shengbiao, He, Hao, Yang, Qi, Rang, Jie, Quan, Meifang, Xia, Liqiu, Ding, Xuezhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595308/
https://www.ncbi.nlm.nih.gov/pubmed/26438125
http://dx.doi.org/10.1186/s12934-015-0339-9
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author Liu, Xuemei
Zuo, Mingxing
Wang, Ting
Sun, Yunjun
Liu, Shuang
Hu, Shengbiao
He, Hao
Yang, Qi
Rang, Jie
Quan, Meifang
Xia, Liqiu
Ding, Xuezhi
author_facet Liu, Xuemei
Zuo, Mingxing
Wang, Ting
Sun, Yunjun
Liu, Shuang
Hu, Shengbiao
He, Hao
Yang, Qi
Rang, Jie
Quan, Meifang
Xia, Liqiu
Ding, Xuezhi
author_sort Liu, Xuemei
collection PubMed
description BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported. RESULTS: The ICP are mainly composed of Cry1Ca, Cry1Ac, and Cry1Da, which have molecular weights of about 130 kDa. ICP production was most efficient when Cu(2+) was added at concentrations ranging from 10(−6) to 10(−4) mol/L at an initial pH of 8.0. Addition of Cu(2+) also evidently increased the toxicity of fermentation broth to Spodoptera exigua and Helicoverpa armigera. After analyzing changes in proteome and fermentation parameters caused by Cu(2+) addition, we propose that Cu(2+) increases PhaR expression and consequently changes the carbon flow. More carbon sources was used to produce intracellular poly-β-hydroxybutyrate (PHB). Increases in PHB as a storage material bring about increases of ICP production. CONCLUSIONS: Bacillus thuringiensis X022 mainly expresses Cry1Ca, Cry1Ac, and Cry1Da. Cu(2+) increases the expression of Cry1Da, Cry1Ca, and also enhances the toxicity of fermentation broth to S. exigua and H. armigera. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0339-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-45953082015-10-08 Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022 Liu, Xuemei Zuo, Mingxing Wang, Ting Sun, Yunjun Liu, Shuang Hu, Shengbiao He, Hao Yang, Qi Rang, Jie Quan, Meifang Xia, Liqiu Ding, Xuezhi Microb Cell Fact Research BACKGROUND: Bacillus thuringiensis X022, a novel strain isolated from soil in China, produces diamond-shaped parasporal crystals. Specific mineral nutrients, such as Mg, Cu, and Mn, influence insecticidal crystal proteins (ICP) expression and the effects of these elements vary significantly. However, the molecular mechanisms of the effects caused by mineral elements have yet to be reported. RESULTS: The ICP are mainly composed of Cry1Ca, Cry1Ac, and Cry1Da, which have molecular weights of about 130 kDa. ICP production was most efficient when Cu(2+) was added at concentrations ranging from 10(−6) to 10(−4) mol/L at an initial pH of 8.0. Addition of Cu(2+) also evidently increased the toxicity of fermentation broth to Spodoptera exigua and Helicoverpa armigera. After analyzing changes in proteome and fermentation parameters caused by Cu(2+) addition, we propose that Cu(2+) increases PhaR expression and consequently changes the carbon flow. More carbon sources was used to produce intracellular poly-β-hydroxybutyrate (PHB). Increases in PHB as a storage material bring about increases of ICP production. CONCLUSIONS: Bacillus thuringiensis X022 mainly expresses Cry1Ca, Cry1Ac, and Cry1Da. Cu(2+) increases the expression of Cry1Da, Cry1Ca, and also enhances the toxicity of fermentation broth to S. exigua and H. armigera. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0339-9) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-05 /pmc/articles/PMC4595308/ /pubmed/26438125 http://dx.doi.org/10.1186/s12934-015-0339-9 Text en © Liu et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liu, Xuemei
Zuo, Mingxing
Wang, Ting
Sun, Yunjun
Liu, Shuang
Hu, Shengbiao
He, Hao
Yang, Qi
Rang, Jie
Quan, Meifang
Xia, Liqiu
Ding, Xuezhi
Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title_full Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title_fullStr Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title_full_unstemmed Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title_short Proteomic analysis of the influence of Cu(2+) on the crystal protein production of Bacillus thuringiensis X022
title_sort proteomic analysis of the influence of cu(2+) on the crystal protein production of bacillus thuringiensis x022
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595308/
https://www.ncbi.nlm.nih.gov/pubmed/26438125
http://dx.doi.org/10.1186/s12934-015-0339-9
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