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Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast

RNA interference (RNAi) is a widespread gene-silencing mechanism and is required for heterochromatin assembly in a variety of organisms. The RNA-induced transcriptional silencing complex (RITS), composed of Ago1, Tas3 and Chp1, is a key component of RNAi machinery in fission yeast that connects shor...

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Autores principales: Deng, Xiaolong, Zhou, Huan, Zhang, Guiping, Wang, Wenchao, Mao, Langyong, Zhou, Xing, Yu, Yao, Lu, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595766/
https://www.ncbi.nlm.nih.gov/pubmed/26443059
http://dx.doi.org/10.1038/srep14707
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author Deng, Xiaolong
Zhou, Huan
Zhang, Guiping
Wang, Wenchao
Mao, Langyong
Zhou, Xing
Yu, Yao
Lu, Hong
author_facet Deng, Xiaolong
Zhou, Huan
Zhang, Guiping
Wang, Wenchao
Mao, Langyong
Zhou, Xing
Yu, Yao
Lu, Hong
author_sort Deng, Xiaolong
collection PubMed
description RNA interference (RNAi) is a widespread gene-silencing mechanism and is required for heterochromatin assembly in a variety of organisms. The RNA-induced transcriptional silencing complex (RITS), composed of Ago1, Tas3 and Chp1, is a key component of RNAi machinery in fission yeast that connects short interference RNA (siRNA) and heterochromatin formation. However, the process by which RITS is assembled is not well understood. Here, we identified Sgf73, a subunit of the SAGA co-transcriptional complex, is required for pericentromeric heterochromatin silencing and the generation of siRNA. This novel role of Sgf73 is independent of enzymatic activities or structural integrity of SAGA. Instead, Sgf73 is physically associated with Ago1 and Chp1. The interactions among the subunits of the RITS, including those between Tas3 and Chp1, between Chp1 and Ago1, between Ago1 and Tas3, were all impaired by the deletion of sgf73(+). Consistently, the recruitment of Ago1 and Chp1 to the pericentromeric region was abolished in sgf73Δ cells. Our study unveils a moonlighting function of a SAGA subunit. It suggests Sgf73 is a novel factor that promotes assembly of RITS and RNAi-mediated heterochromatin formation.
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spelling pubmed-45957662015-10-13 Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast Deng, Xiaolong Zhou, Huan Zhang, Guiping Wang, Wenchao Mao, Langyong Zhou, Xing Yu, Yao Lu, Hong Sci Rep Article RNA interference (RNAi) is a widespread gene-silencing mechanism and is required for heterochromatin assembly in a variety of organisms. The RNA-induced transcriptional silencing complex (RITS), composed of Ago1, Tas3 and Chp1, is a key component of RNAi machinery in fission yeast that connects short interference RNA (siRNA) and heterochromatin formation. However, the process by which RITS is assembled is not well understood. Here, we identified Sgf73, a subunit of the SAGA co-transcriptional complex, is required for pericentromeric heterochromatin silencing and the generation of siRNA. This novel role of Sgf73 is independent of enzymatic activities or structural integrity of SAGA. Instead, Sgf73 is physically associated with Ago1 and Chp1. The interactions among the subunits of the RITS, including those between Tas3 and Chp1, between Chp1 and Ago1, between Ago1 and Tas3, were all impaired by the deletion of sgf73(+). Consistently, the recruitment of Ago1 and Chp1 to the pericentromeric region was abolished in sgf73Δ cells. Our study unveils a moonlighting function of a SAGA subunit. It suggests Sgf73 is a novel factor that promotes assembly of RITS and RNAi-mediated heterochromatin formation. Nature Publishing Group 2015-10-07 /pmc/articles/PMC4595766/ /pubmed/26443059 http://dx.doi.org/10.1038/srep14707 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Deng, Xiaolong
Zhou, Huan
Zhang, Guiping
Wang, Wenchao
Mao, Langyong
Zhou, Xing
Yu, Yao
Lu, Hong
Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title_full Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title_fullStr Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title_full_unstemmed Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title_short Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast
title_sort sgf73, a subunit of saga complex, is required for the assembly of rits complex in fission yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595766/
https://www.ncbi.nlm.nih.gov/pubmed/26443059
http://dx.doi.org/10.1038/srep14707
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