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Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis
Lipases (EC 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting applications of lipase is in the biofuel industry for biodiesel product...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595793/ https://www.ncbi.nlm.nih.gov/pubmed/26500628 http://dx.doi.org/10.3389/fmicb.2015.01083 |
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author | Vici, Ana C. da Cruz, Andrezza F. Facchini, Fernanda D. A. de Carvalho, Caio C. Pereira, Marita G. Fonseca-Maldonado, Raquel Ward, Richard J. Pessela, Benevides C. Fernandez-Lorente, Gloria Torres, Fernando A. G. Jorge, João A. Polizeli, Maria L. T. M. |
author_facet | Vici, Ana C. da Cruz, Andrezza F. Facchini, Fernanda D. A. de Carvalho, Caio C. Pereira, Marita G. Fonseca-Maldonado, Raquel Ward, Richard J. Pessela, Benevides C. Fernandez-Lorente, Gloria Torres, Fernando A. G. Jorge, João A. Polizeli, Maria L. T. M. |
author_sort | Vici, Ana C. |
collection | PubMed |
description | Lipases (EC 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting applications of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol) transesterification. Entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. The present work reports the heterologous expression and biochemical characterization of a novel Beauveria bassiana lipase with potential for biodiesel production. The His-tagged B. bassiana lipase A (BbLA) was produced in Komagataella pastoris in buffered methanol medium (BMM) induced with 1% methanol at 30°C. Purified BbLA was activated with 0.05% Triton X-100 and presented optimum activity at pH 6.0 and 50°C. N-glycosylation of the recombinant BbLA accounts for 31.5% of its molecular weight. Circular dichroism and molecular modeling confirmed a structure composed of α-helix and β-sheet, similar to α/β hydrolases. Immobilized BbLA was able to promote transesterification reactions in fish oil, demonstrating potential for biodiesel production. BbLA was successfully produced in K. pastoris and shows potential use for biodiesel production by the ethanolysis reaction. |
format | Online Article Text |
id | pubmed-4595793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45957932015-10-23 Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis Vici, Ana C. da Cruz, Andrezza F. Facchini, Fernanda D. A. de Carvalho, Caio C. Pereira, Marita G. Fonseca-Maldonado, Raquel Ward, Richard J. Pessela, Benevides C. Fernandez-Lorente, Gloria Torres, Fernando A. G. Jorge, João A. Polizeli, Maria L. T. M. Front Microbiol Microbiology Lipases (EC 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting applications of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol) transesterification. Entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. The present work reports the heterologous expression and biochemical characterization of a novel Beauveria bassiana lipase with potential for biodiesel production. The His-tagged B. bassiana lipase A (BbLA) was produced in Komagataella pastoris in buffered methanol medium (BMM) induced with 1% methanol at 30°C. Purified BbLA was activated with 0.05% Triton X-100 and presented optimum activity at pH 6.0 and 50°C. N-glycosylation of the recombinant BbLA accounts for 31.5% of its molecular weight. Circular dichroism and molecular modeling confirmed a structure composed of α-helix and β-sheet, similar to α/β hydrolases. Immobilized BbLA was able to promote transesterification reactions in fish oil, demonstrating potential for biodiesel production. BbLA was successfully produced in K. pastoris and shows potential use for biodiesel production by the ethanolysis reaction. Frontiers Media S.A. 2015-10-07 /pmc/articles/PMC4595793/ /pubmed/26500628 http://dx.doi.org/10.3389/fmicb.2015.01083 Text en Copyright © 2015 Vici, da Cruz, Facchini, de Carvalho, Pereira, Fonseca-Maldonado, Ward, Pessela, Fernandez-Lorente, Torres, Jorge and Polizeli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Vici, Ana C. da Cruz, Andrezza F. Facchini, Fernanda D. A. de Carvalho, Caio C. Pereira, Marita G. Fonseca-Maldonado, Raquel Ward, Richard J. Pessela, Benevides C. Fernandez-Lorente, Gloria Torres, Fernando A. G. Jorge, João A. Polizeli, Maria L. T. M. Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title | Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title_full | Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title_fullStr | Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title_full_unstemmed | Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title_short | Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis |
title_sort | beauveria bassiana lipase a expressed in komagataella (pichia) pastoris with potential for biodiesel catalysis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595793/ https://www.ncbi.nlm.nih.gov/pubmed/26500628 http://dx.doi.org/10.3389/fmicb.2015.01083 |
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