Real-time multi-view deconvolution

Summary: In light-sheet microscopy, overall image content and resolution are improved by acquiring and fusing multiple views of the sample from different directions. State-of-the-art multi-view (MV) deconvolution simultaneously fuses and deconvolves the images in 3D, but processing takes a multiple of the acquisition time and constitutes the bottleneck in the imaging pipeline. Here, we show that MV deconvolution in 3D can finally be achieved in real-time by processing cross-sectional planes individually on the massively parallel architecture of a graphics processing unit (GPU). Our approximation is valid in the typical case where the rotation axis lies in the imaging plane. Availability and implementation: Source code and binaries are available on github (https://github.com/bene51/), native code under the repository ‘gpu_deconvolution’, Java wrappers implementing Fiji plugins under ‘SPIM_Reconstruction_Cuda’. Contact: bschmid@mpi-cbg.de or huisken@mpi-cbg.de Supplementary information: Supplementary data are available at Bioinformatics online.