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Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses
Citrus canker, caused by the bacterial pathogen Xanthomonas citri ssp. citri (Xcc), has been attributed to millions of dollars in loss or damage to commercial citrus crops in subtropical production areas of the world. Since identification of resistant plants is one of the most effective methods of d...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595992/ https://www.ncbi.nlm.nih.gov/pubmed/26504581 http://dx.doi.org/10.1038/hortres.2015.42 |
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author | Pitino, Marco Armstrong, Cheryl M Duan, Yongping |
author_facet | Pitino, Marco Armstrong, Cheryl M Duan, Yongping |
author_sort | Pitino, Marco |
collection | PubMed |
description | Citrus canker, caused by the bacterial pathogen Xanthomonas citri ssp. citri (Xcc), has been attributed to millions of dollars in loss or damage to commercial citrus crops in subtropical production areas of the world. Since identification of resistant plants is one of the most effective methods of disease management, the ability to screen for resistant seedlings plays a key role in the production of a long-term solution to canker. Here, an inverse correlation between reactive oxygen species (ROS) production by the plant and the ability of Xcc to grow and form lesions on infected plants is reported. Based on this information, a novel screening method that can rapidly identify citrus seedlings that are less susceptible to early infection by Xcc was devised by measuring ROS accumulation triggered by a 22-amino acid sequence of the conserved N-terminal part of flagellin (flg22) from X. citri ssp. citri (Xcc-flg22). In addition to limiting disease symptoms, ROS production was also correlated with the expression of basal defense-related genes such as the pattern recognition receptors LRR8 and FLS2, the leucine-rich repeat receptor-like protein RLP12, and the defense-related gene PR1, indicating an important role for pathogen-associated molecular pattern-triggered immunity (PTI) in determining resistance to citrus canker. Moreover, the differential expression patterns observed amongst the citrus seedlings demonstrated the existence of genetic variations in the PTI response among citrus species/varieties. |
format | Online Article Text |
id | pubmed-4595992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45959922015-10-26 Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses Pitino, Marco Armstrong, Cheryl M Duan, Yongping Hortic Res Article Citrus canker, caused by the bacterial pathogen Xanthomonas citri ssp. citri (Xcc), has been attributed to millions of dollars in loss or damage to commercial citrus crops in subtropical production areas of the world. Since identification of resistant plants is one of the most effective methods of disease management, the ability to screen for resistant seedlings plays a key role in the production of a long-term solution to canker. Here, an inverse correlation between reactive oxygen species (ROS) production by the plant and the ability of Xcc to grow and form lesions on infected plants is reported. Based on this information, a novel screening method that can rapidly identify citrus seedlings that are less susceptible to early infection by Xcc was devised by measuring ROS accumulation triggered by a 22-amino acid sequence of the conserved N-terminal part of flagellin (flg22) from X. citri ssp. citri (Xcc-flg22). In addition to limiting disease symptoms, ROS production was also correlated with the expression of basal defense-related genes such as the pattern recognition receptors LRR8 and FLS2, the leucine-rich repeat receptor-like protein RLP12, and the defense-related gene PR1, indicating an important role for pathogen-associated molecular pattern-triggered immunity (PTI) in determining resistance to citrus canker. Moreover, the differential expression patterns observed amongst the citrus seedlings demonstrated the existence of genetic variations in the PTI response among citrus species/varieties. Nature Publishing Group 2015-09-09 /pmc/articles/PMC4595992/ /pubmed/26504581 http://dx.doi.org/10.1038/hortres.2015.42 Text en Copyright © 2015 Nanjing Agricultural University http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Pitino, Marco Armstrong, Cheryl M Duan, Yongping Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title | Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title_full | Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title_fullStr | Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title_full_unstemmed | Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title_short | Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
title_sort | rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4595992/ https://www.ncbi.nlm.nih.gov/pubmed/26504581 http://dx.doi.org/10.1038/hortres.2015.42 |
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