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A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans
Caenorhabditis elegans postembryonic development consists of four discrete larval stages separated by molts. Typically, the speed of progression through these larval stages is investigated by visual inspection of the molting process. Here, we describe an automated method to monitor the timing of the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596660/ https://www.ncbi.nlm.nih.gov/pubmed/26294666 http://dx.doi.org/10.1534/genetics.115.179242 |
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author | Olmedo, María Geibel, Mirjam Artal-Sanz, Marta Merrow, Martha |
author_facet | Olmedo, María Geibel, Mirjam Artal-Sanz, Marta Merrow, Martha |
author_sort | Olmedo, María |
collection | PubMed |
description | Caenorhabditis elegans postembryonic development consists of four discrete larval stages separated by molts. Typically, the speed of progression through these larval stages is investigated by visual inspection of the molting process. Here, we describe an automated method to monitor the timing of these discrete phases of C. elegans maturation, from the first larval stage through adulthood, using bioluminescence. The method was validated with a lin-42 mutant strain that shows delayed development relative to wild-type animals and with a daf-2 mutant that shows an extended second larval stage. This new method is inherently high-throughput and will finally allow dissecting the molecular machinery governing the speed of the developmental clock, which has so far been hampered by the lack of a method suitable for genetic screens. |
format | Online Article Text |
id | pubmed-4596660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-45966602015-10-19 A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans Olmedo, María Geibel, Mirjam Artal-Sanz, Marta Merrow, Martha Genetics Communications Caenorhabditis elegans postembryonic development consists of four discrete larval stages separated by molts. Typically, the speed of progression through these larval stages is investigated by visual inspection of the molting process. Here, we describe an automated method to monitor the timing of these discrete phases of C. elegans maturation, from the first larval stage through adulthood, using bioluminescence. The method was validated with a lin-42 mutant strain that shows delayed development relative to wild-type animals and with a daf-2 mutant that shows an extended second larval stage. This new method is inherently high-throughput and will finally allow dissecting the molecular machinery governing the speed of the developmental clock, which has so far been hampered by the lack of a method suitable for genetic screens. Genetics Society of America 2015-10 2015-08-20 /pmc/articles/PMC4596660/ /pubmed/26294666 http://dx.doi.org/10.1534/genetics.115.179242 Text en Copyright © 2015 by the Genetics Society of America Available freely online through the author-supported open access option. |
spellingShingle | Communications Olmedo, María Geibel, Mirjam Artal-Sanz, Marta Merrow, Martha A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title | A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title_full | A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title_fullStr | A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title_full_unstemmed | A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title_short | A High-Throughput Method for the Analysis of Larval Developmental Phenotypes in Caenorhabditis elegans |
title_sort | high-throughput method for the analysis of larval developmental phenotypes in caenorhabditis elegans |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596660/ https://www.ncbi.nlm.nih.gov/pubmed/26294666 http://dx.doi.org/10.1534/genetics.115.179242 |
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