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Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis

Hydrogen gas (H(2)) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H(2) in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H(2) in...

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Autores principales: Wu, Qi, Su, Nana, Chen, Qin, Shen, Wenbiao, Shen, Zhenguo, Xia, Yan, Cui, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596834/
https://www.ncbi.nlm.nih.gov/pubmed/26445361
http://dx.doi.org/10.1371/journal.pone.0139956
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author Wu, Qi
Su, Nana
Chen, Qin
Shen, Wenbiao
Shen, Zhenguo
Xia, Yan
Cui, Jin
author_facet Wu, Qi
Su, Nana
Chen, Qin
Shen, Wenbiao
Shen, Zhenguo
Xia, Yan
Cui, Jin
author_sort Wu, Qi
collection PubMed
description Hydrogen gas (H(2)) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H(2) in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H(2) in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl(2) and 50%-saturated HRW induced an increase of endogenous H(2) in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H(2) might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis.
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spelling pubmed-45968342015-10-20 Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis Wu, Qi Su, Nana Chen, Qin Shen, Wenbiao Shen, Zhenguo Xia, Yan Cui, Jin PLoS One Research Article Hydrogen gas (H(2)) was recently proposed as a therapeutic antioxidant and signaling molecule in clinical trials. However, the underlying physiological roles of H(2) in plants remain unclear. In the present study, hydrogen-rich water (HRW) was used to characterize the physiological roles of H(2) in enhancing the tolerance of Brassica campestris against cadmium (Cd). The results showed that both 50 μM CdCl(2) and 50%-saturated HRW induced an increase of endogenous H(2) in Brassica campestris seedlings, and HRW alleviated Cd toxicity related to growth inhibition and oxidative damage. Seedlings supplied with HRW exhibited increased root length and reduced lipid peroxidation, similar to plants receiving GSH post-treatment. Additionally, seedlings post-treated with HRW accumulated higher levels of reduced glutathione (GSH) and ascorbic acid (AsA) and showed increased GST and GPX activities in roots. Molecular evidence illustrated that the expression of genes such as GS, GR1 and GR2, which were down-regulated following the addition of Cd, GSH or BSO, could be reversed to varying degrees by the addition of HRW. Based on these results, it could be proposed that H(2) might be an important regulator for enhancing the tolerance of Brassica campestris seedlings against Cd, mainly by governing reduced glutathione homeostasis. Public Library of Science 2015-10-07 /pmc/articles/PMC4596834/ /pubmed/26445361 http://dx.doi.org/10.1371/journal.pone.0139956 Text en © 2015 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Qi
Su, Nana
Chen, Qin
Shen, Wenbiao
Shen, Zhenguo
Xia, Yan
Cui, Jin
Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title_full Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title_fullStr Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title_full_unstemmed Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title_short Cadmium-Induced Hydrogen Accumulation Is Involved in Cadmium Tolerance in Brassica campestris by Reestablishment of Reduced Glutathione Homeostasis
title_sort cadmium-induced hydrogen accumulation is involved in cadmium tolerance in brassica campestris by reestablishment of reduced glutathione homeostasis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596834/
https://www.ncbi.nlm.nih.gov/pubmed/26445361
http://dx.doi.org/10.1371/journal.pone.0139956
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