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Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
[Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NI...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597160/ https://www.ncbi.nlm.nih.gov/pubmed/26457250 http://dx.doi.org/10.15171/bi.2015.16 |
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author | Khodadadi, Roghayeh Khajeali, Azim Farajollahi, Ali Reza Hajalioghli, Parisa Raeisi, Noorallah |
author_facet | Khodadadi, Roghayeh Khajeali, Azim Farajollahi, Ali Reza Hajalioghli, Parisa Raeisi, Noorallah |
author_sort | Khodadadi, Roghayeh |
collection | PubMed |
description | [Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NIPAM polymer gel was prepared from non-electrophoresis grade BIS and the relaxation rate (R(2)) was measured by MR imaging after exposing the gel to gamma radiation from Co-60 source. To compare the response of this gel with the one that contains electrophoresis grade BIS, two sets of NIPAM gel were prepared using electrophoresis and non-electrophoresis BIS and irradiated to different gamma doses. Results: It was found that the dose–response of NIPAM gel made from the non-electrophoresis grade BIS is coincident with that of electrophoresis grade BIS. Conclusion:Taken all, it can be concluded that the non-electrophoresis grade BIS not only is a suitable alternative for the electrophoresis grade BIS but also reduces the cost of gel due to its lower price. |
format | Online Article Text |
id | pubmed-4597160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-45971602015-10-09 Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation Khodadadi, Roghayeh Khajeali, Azim Farajollahi, Ali Reza Hajalioghli, Parisa Raeisi, Noorallah Bioimpacts Original Article [Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NIPAM polymer gel was prepared from non-electrophoresis grade BIS and the relaxation rate (R(2)) was measured by MR imaging after exposing the gel to gamma radiation from Co-60 source. To compare the response of this gel with the one that contains electrophoresis grade BIS, two sets of NIPAM gel were prepared using electrophoresis and non-electrophoresis BIS and irradiated to different gamma doses. Results: It was found that the dose–response of NIPAM gel made from the non-electrophoresis grade BIS is coincident with that of electrophoresis grade BIS. Conclusion:Taken all, it can be concluded that the non-electrophoresis grade BIS not only is a suitable alternative for the electrophoresis grade BIS but also reduces the cost of gel due to its lower price. Tabriz University of Medical Sciences 2015 2015-07-09 /pmc/articles/PMC4597160/ /pubmed/26457250 http://dx.doi.org/10.15171/bi.2015.16 Text en © 2015 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Khodadadi, Roghayeh Khajeali, Azim Farajollahi, Ali Reza Hajalioghli, Parisa Raeisi, Noorallah Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title | Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title_full | Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title_fullStr | Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title_full_unstemmed | Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title_short | Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation |
title_sort | comparison of non-electrophoresis grade with electrophoresis grade bis in nipam polymer gel preparation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597160/ https://www.ncbi.nlm.nih.gov/pubmed/26457250 http://dx.doi.org/10.15171/bi.2015.16 |
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