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Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation

[Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NI...

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Autores principales: Khodadadi, Roghayeh, Khajeali, Azim, Farajollahi, Ali Reza, Hajalioghli, Parisa, Raeisi, Noorallah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597160/
https://www.ncbi.nlm.nih.gov/pubmed/26457250
http://dx.doi.org/10.15171/bi.2015.16
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author Khodadadi, Roghayeh
Khajeali, Azim
Farajollahi, Ali Reza
Hajalioghli, Parisa
Raeisi, Noorallah
author_facet Khodadadi, Roghayeh
Khajeali, Azim
Farajollahi, Ali Reza
Hajalioghli, Parisa
Raeisi, Noorallah
author_sort Khodadadi, Roghayeh
collection PubMed
description [Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NIPAM polymer gel was prepared from non-electrophoresis grade BIS and the relaxation rate (R(2)) was measured by MR imaging after exposing the gel to gamma radiation from Co-60 source. To compare the response of this gel with the one that contains electrophoresis grade BIS, two sets of NIPAM gel were prepared using electrophoresis and non-electrophoresis BIS and irradiated to different gamma doses. Results: It was found that the dose–response of NIPAM gel made from the non-electrophoresis grade BIS is coincident with that of electrophoresis grade BIS. Conclusion:Taken all, it can be concluded that the non-electrophoresis grade BIS not only is a suitable alternative for the electrophoresis grade BIS but also reduces the cost of gel due to its lower price.
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spelling pubmed-45971602015-10-09 Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation Khodadadi, Roghayeh Khajeali, Azim Farajollahi, Ali Reza Hajalioghli, Parisa Raeisi, Noorallah Bioimpacts Original Article [Image: see text] Introduction:The main objective of this study was to investigate the possibility of replacing electrophoresis cross-linker with non-electrophoresis N, N′-methylenebisacrylamide (BIS) in N-isopropyl acrylamide (NIPAM) polymer gel and its possible effect on dose response. Methods: NIPAM polymer gel was prepared from non-electrophoresis grade BIS and the relaxation rate (R(2)) was measured by MR imaging after exposing the gel to gamma radiation from Co-60 source. To compare the response of this gel with the one that contains electrophoresis grade BIS, two sets of NIPAM gel were prepared using electrophoresis and non-electrophoresis BIS and irradiated to different gamma doses. Results: It was found that the dose–response of NIPAM gel made from the non-electrophoresis grade BIS is coincident with that of electrophoresis grade BIS. Conclusion:Taken all, it can be concluded that the non-electrophoresis grade BIS not only is a suitable alternative for the electrophoresis grade BIS but also reduces the cost of gel due to its lower price. Tabriz University of Medical Sciences 2015 2015-07-09 /pmc/articles/PMC4597160/ /pubmed/26457250 http://dx.doi.org/10.15171/bi.2015.16 Text en © 2015 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Khodadadi, Roghayeh
Khajeali, Azim
Farajollahi, Ali Reza
Hajalioghli, Parisa
Raeisi, Noorallah
Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title_full Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title_fullStr Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title_full_unstemmed Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title_short Comparison of non-electrophoresis grade with electrophoresis grade BIS in NIPAM polymer gel preparation
title_sort comparison of non-electrophoresis grade with electrophoresis grade bis in nipam polymer gel preparation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597160/
https://www.ncbi.nlm.nih.gov/pubmed/26457250
http://dx.doi.org/10.15171/bi.2015.16
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