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In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
[Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Tabriz University of Medical Sciences
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597161/ https://www.ncbi.nlm.nih.gov/pubmed/26457251 http://dx.doi.org/10.15171/bi.2015.17 |
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author | Asnaashari, Solmaz Heshmati Afshar, Fariba Ebrahimi, Atefeh Bamdad Moghadam, Sedigheh Delazar, Abbas |
author_facet | Asnaashari, Solmaz Heshmati Afshar, Fariba Ebrahimi, Atefeh Bamdad Moghadam, Sedigheh Delazar, Abbas |
author_sort | Asnaashari, Solmaz |
collection | PubMed |
description | [Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes of Eremostachys macrophylla Montbr. & Auch., were screened for their antimalarial properties by cell-free β-hematin formation assay. Results: Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC(50) values of 0.797 ± 0.016 mg/mL in aerial parts and 0.324 ± 0.039 mg/mL in rhizomes compared to positive control (Chloroquine, IC(50) = 0.014 ± 0.003 mg/mL, IC(90) = 0.163 ± 0.004 mg/mL). Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Sixty percent ethyl acetate/n-hexane fraction showed considerable antimalarial activity with IC(50) value of 0.047 ± 0.0003 mg/mL. Conclusion: From 6 extracts with different polarity of E. macrophylla,s aerial parts and rhizomes, the DCM extract of both parts were the most active extract in this assay. The preliminary phytochemical study on the VLC fractions of the most potent part persuades us to focus on purifying the active components of these extracts and to conduct further investigation towards in vivo evaluation. |
format | Online Article Text |
id | pubmed-4597161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-45971612015-10-09 In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch. Asnaashari, Solmaz Heshmati Afshar, Fariba Ebrahimi, Atefeh Bamdad Moghadam, Sedigheh Delazar, Abbas Bioimpacts Original Article [Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes of Eremostachys macrophylla Montbr. & Auch., were screened for their antimalarial properties by cell-free β-hematin formation assay. Results: Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC(50) values of 0.797 ± 0.016 mg/mL in aerial parts and 0.324 ± 0.039 mg/mL in rhizomes compared to positive control (Chloroquine, IC(50) = 0.014 ± 0.003 mg/mL, IC(90) = 0.163 ± 0.004 mg/mL). Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Sixty percent ethyl acetate/n-hexane fraction showed considerable antimalarial activity with IC(50) value of 0.047 ± 0.0003 mg/mL. Conclusion: From 6 extracts with different polarity of E. macrophylla,s aerial parts and rhizomes, the DCM extract of both parts were the most active extract in this assay. The preliminary phytochemical study on the VLC fractions of the most potent part persuades us to focus on purifying the active components of these extracts and to conduct further investigation towards in vivo evaluation. Tabriz University of Medical Sciences 2015 2015-08-19 /pmc/articles/PMC4597161/ /pubmed/26457251 http://dx.doi.org/10.15171/bi.2015.17 Text en © 2015 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article Asnaashari, Solmaz Heshmati Afshar, Fariba Ebrahimi, Atefeh Bamdad Moghadam, Sedigheh Delazar, Abbas In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch. |
title |
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
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title_full |
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
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title_fullStr |
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
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title_full_unstemmed |
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
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title_short |
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
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title_sort | in vitro antimalarial activity of different extracts of eremostachys macrophylla montbr. & auch. |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597161/ https://www.ncbi.nlm.nih.gov/pubmed/26457251 http://dx.doi.org/10.15171/bi.2015.17 |
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