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In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.

[Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes...

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Autores principales: Asnaashari, Solmaz, Heshmati Afshar, Fariba, Ebrahimi, Atefeh, Bamdad Moghadam, Sedigheh, Delazar, Abbas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597161/
https://www.ncbi.nlm.nih.gov/pubmed/26457251
http://dx.doi.org/10.15171/bi.2015.17
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author Asnaashari, Solmaz
Heshmati Afshar, Fariba
Ebrahimi, Atefeh
Bamdad Moghadam, Sedigheh
Delazar, Abbas
author_facet Asnaashari, Solmaz
Heshmati Afshar, Fariba
Ebrahimi, Atefeh
Bamdad Moghadam, Sedigheh
Delazar, Abbas
author_sort Asnaashari, Solmaz
collection PubMed
description [Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes of Eremostachys macrophylla Montbr. & Auch., were screened for their antimalarial properties by cell-free β-hematin formation assay. Results: Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC(50) values of 0.797 ± 0.016 mg/mL in aerial parts and 0.324 ± 0.039 mg/mL in rhizomes compared to positive control (Chloroquine, IC(50) = 0.014 ± 0.003 mg/mL, IC(90) = 0.163 ± 0.004 mg/mL). Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Sixty percent ethyl acetate/n-hexane fraction showed considerable antimalarial activity with IC(50) value of 0.047 ± 0.0003 mg/mL. Conclusion: From 6 extracts with different polarity of E. macrophylla,s aerial parts and rhizomes, the DCM extract of both parts were the most active extract in this assay. The preliminary phytochemical study on the VLC fractions of the most potent part persuades us to focus on purifying the active components of these extracts and to conduct further investigation towards in vivo evaluation.
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spelling pubmed-45971612015-10-09 In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch. Asnaashari, Solmaz Heshmati Afshar, Fariba Ebrahimi, Atefeh Bamdad Moghadam, Sedigheh Delazar, Abbas Bioimpacts Original Article [Image: see text] Introduction:The risk of drug resistance and the use of medicinal plants in malaria prevention and treatment have led to the search for new antimalarial compounds with natural origin. Methods:In the current study, six extracts with different polarity from aerial parts and rhizomes of Eremostachys macrophylla Montbr. & Auch., were screened for their antimalarial properties by cell-free β-hematin formation assay. Results: Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC(50) values of 0.797 ± 0.016 mg/mL in aerial parts and 0.324 ± 0.039 mg/mL in rhizomes compared to positive control (Chloroquine, IC(50) = 0.014 ± 0.003 mg/mL, IC(90) = 0.163 ± 0.004 mg/mL). Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Sixty percent ethyl acetate/n-hexane fraction showed considerable antimalarial activity with IC(50) value of 0.047 ± 0.0003 mg/mL. Conclusion: From 6 extracts with different polarity of E. macrophylla,s aerial parts and rhizomes, the DCM extract of both parts were the most active extract in this assay. The preliminary phytochemical study on the VLC fractions of the most potent part persuades us to focus on purifying the active components of these extracts and to conduct further investigation towards in vivo evaluation. Tabriz University of Medical Sciences 2015 2015-08-19 /pmc/articles/PMC4597161/ /pubmed/26457251 http://dx.doi.org/10.15171/bi.2015.17 Text en © 2015 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Asnaashari, Solmaz
Heshmati Afshar, Fariba
Ebrahimi, Atefeh
Bamdad Moghadam, Sedigheh
Delazar, Abbas
In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title_full In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title_fullStr In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title_full_unstemmed In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title_short In vitro antimalarial activity of different extracts of Eremostachys macrophylla Montbr. & Auch.
title_sort in vitro antimalarial activity of different extracts of eremostachys macrophylla montbr. & auch.
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597161/
https://www.ncbi.nlm.nih.gov/pubmed/26457251
http://dx.doi.org/10.15171/bi.2015.17
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