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Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways

Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the...

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Autores principales: Liang, Fang, Han, Yuxiang, Gao, Hao, Xin, Shengchang, Chen, Shaodan, Wang, Nan, Qin, Wei, Zhong, Hanbing, Lin, Shuo, Yao, Xinsheng, Li, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597183/
https://www.ncbi.nlm.nih.gov/pubmed/26446489
http://dx.doi.org/10.1038/srep14468
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author Liang, Fang
Han, Yuxiang
Gao, Hao
Xin, Shengchang
Chen, Shaodan
Wang, Nan
Qin, Wei
Zhong, Hanbing
Lin, Shuo
Yao, Xinsheng
Li, Song
author_facet Liang, Fang
Han, Yuxiang
Gao, Hao
Xin, Shengchang
Chen, Shaodan
Wang, Nan
Qin, Wei
Zhong, Hanbing
Lin, Shuo
Yao, Xinsheng
Li, Song
author_sort Liang, Fang
collection PubMed
description Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the growth of intersegmental vessels in the zebrafish vasculature. Bioassay-guided isolation of DYVE-D3 indicates that the flavonoid kaempferol was the active substance. Kaempferol also inhibited the proliferation and migration of HUVECs in vitro. Furthermore, we found that kaempferol suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows that the construction of fine fraction libraries allows efficient identification of active substances from natural products.
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spelling pubmed-45971832015-10-13 Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways Liang, Fang Han, Yuxiang Gao, Hao Xin, Shengchang Chen, Shaodan Wang, Nan Qin, Wei Zhong, Hanbing Lin, Shuo Yao, Xinsheng Li, Song Sci Rep Article Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the growth of intersegmental vessels in the zebrafish vasculature. Bioassay-guided isolation of DYVE-D3 indicates that the flavonoid kaempferol was the active substance. Kaempferol also inhibited the proliferation and migration of HUVECs in vitro. Furthermore, we found that kaempferol suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows that the construction of fine fraction libraries allows efficient identification of active substances from natural products. Nature Publishing Group 2015-10-08 /pmc/articles/PMC4597183/ /pubmed/26446489 http://dx.doi.org/10.1038/srep14468 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liang, Fang
Han, Yuxiang
Gao, Hao
Xin, Shengchang
Chen, Shaodan
Wang, Nan
Qin, Wei
Zhong, Hanbing
Lin, Shuo
Yao, Xinsheng
Li, Song
Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title_full Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title_fullStr Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title_full_unstemmed Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title_short Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
title_sort kaempferol identified by zebrafish assay and fine fractionations strategy from dysosma versipellis inhibits angiogenesis through vegf and fgf pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597183/
https://www.ncbi.nlm.nih.gov/pubmed/26446489
http://dx.doi.org/10.1038/srep14468
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