Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength

Luminescence thermometry usually suffer from cellular complexity of the biochemical environment (such as pH and ionic strength), and thus the accuracy and reliability of the determined intracellular temperature are directly affected. Herein, a photoluminescent nanothermometer composed of polymer enc...

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Detalles Bibliográficos
Autores principales: Liu, Helin, Fan, Yanyan, Wang, Jianhai, Song, Zhongsen, Shi, Hao, Han, Rongcheng, Sha, Yinlin, Jiang, Yuqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597201/
https://www.ncbi.nlm.nih.gov/pubmed/26445905
http://dx.doi.org/10.1038/srep14879
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author Liu, Helin
Fan, Yanyan
Wang, Jianhai
Song, Zhongsen
Shi, Hao
Han, Rongcheng
Sha, Yinlin
Jiang, Yuqiang
author_facet Liu, Helin
Fan, Yanyan
Wang, Jianhai
Song, Zhongsen
Shi, Hao
Han, Rongcheng
Sha, Yinlin
Jiang, Yuqiang
author_sort Liu, Helin
collection PubMed
description Luminescence thermometry usually suffer from cellular complexity of the biochemical environment (such as pH and ionic strength), and thus the accuracy and reliability of the determined intracellular temperature are directly affected. Herein, a photoluminescent nanothermometer composed of polymer encapsulated quantum dots (P-QD) has been developed. And the prepared nanothermometer exhibits some advantages: such as non-sensitivity to pH and ionic strength, as well as high detection sensitivity and ultrahigh reversibility. The intracellular temperature was accurately determined under physiological conditions with different pH and ionic strength, and direct measurement of thermogenesis in individual cells has been achieved.
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spelling pubmed-45972012015-10-13 Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength Liu, Helin Fan, Yanyan Wang, Jianhai Song, Zhongsen Shi, Hao Han, Rongcheng Sha, Yinlin Jiang, Yuqiang Sci Rep Article Luminescence thermometry usually suffer from cellular complexity of the biochemical environment (such as pH and ionic strength), and thus the accuracy and reliability of the determined intracellular temperature are directly affected. Herein, a photoluminescent nanothermometer composed of polymer encapsulated quantum dots (P-QD) has been developed. And the prepared nanothermometer exhibits some advantages: such as non-sensitivity to pH and ionic strength, as well as high detection sensitivity and ultrahigh reversibility. The intracellular temperature was accurately determined under physiological conditions with different pH and ionic strength, and direct measurement of thermogenesis in individual cells has been achieved. Nature Publishing Group 2015-10-08 /pmc/articles/PMC4597201/ /pubmed/26445905 http://dx.doi.org/10.1038/srep14879 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Helin
Fan, Yanyan
Wang, Jianhai
Song, Zhongsen
Shi, Hao
Han, Rongcheng
Sha, Yinlin
Jiang, Yuqiang
Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title_full Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title_fullStr Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title_full_unstemmed Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title_short Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength
title_sort intracellular temperature sensing: an ultra-bright luminescent nanothermometer with non-sensitivity to ph and ionic strength
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597201/
https://www.ncbi.nlm.nih.gov/pubmed/26445905
http://dx.doi.org/10.1038/srep14879
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