Cargando…
Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats
Total spleen lymphocytes, lymphocyte proliferation, tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in spleen lymphocyte culture were studied in malnourished Wistar rats fed with goat milk yoghurt. Malnourished rats were created by using standard feed restriction as much as 50% of normal...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Food Science of Animal Resources
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597830/ https://www.ncbi.nlm.nih.gov/pubmed/26760750 http://dx.doi.org/10.5851/kosfa.2014.34.1.88 |
_version_ | 1782394004608909312 |
---|---|
author | Nurliyani, Kandarina, BJ. Istiti Kusuma, Sari Trisnasari, Yunita Dewi |
author_facet | Nurliyani, Kandarina, BJ. Istiti Kusuma, Sari Trisnasari, Yunita Dewi |
author_sort | Nurliyani, |
collection | PubMed |
description | Total spleen lymphocytes, lymphocyte proliferation, tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in spleen lymphocyte culture were studied in malnourished Wistar rats fed with goat milk yoghurt. Malnourished rats were created by using standard feed restriction as much as 50% of normal rats for 21 d. Goat milk yoghurt containing three types of microorganism e.g., Lactobacillus acidophilus, Sterptococcus thermophilus and Bifidobacterium longum derived from Lacto-B culture in powder form. After 21 d, the rats continued to receive restricted feeding and supplemented with goat milk yoghurt for 7 d. Total splenocytes were counted by hemocytometer. Splenocytes proliferation was expressed as stimulation index, whereas the TNF-α and IL-10 of spleen lymphocyte culture were measured by ELISA technique. The total number of splenocytes and stimulation index of splenocytes in moderate malnourished and normal rats supplemented with goat milk yoghurt was not significantly different. The level of TNF-α in the rat supplemented with goat milk yoghurt was lower (p<0.05) than the control group, whereas the level of IL-10 in the rat supplemented with goat milk yoghurt was higher (p<0.05) than the control group. In conclusion, goat milk yoghurt supplementation in malnourished rats could decrease TNF-α as a representation of the proinflammatory cytokine, while it increases IL-10 as a representation of the anti-inflammatory cytokine. |
format | Online Article Text |
id | pubmed-4597830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Food Science of Animal Resources |
record_format | MEDLINE/PubMed |
spelling | pubmed-45978302016-01-04 Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats Nurliyani, Kandarina, BJ. Istiti Kusuma, Sari Trisnasari, Yunita Dewi Korean J Food Sci Anim Resour Article Total spleen lymphocytes, lymphocyte proliferation, tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in spleen lymphocyte culture were studied in malnourished Wistar rats fed with goat milk yoghurt. Malnourished rats were created by using standard feed restriction as much as 50% of normal rats for 21 d. Goat milk yoghurt containing three types of microorganism e.g., Lactobacillus acidophilus, Sterptococcus thermophilus and Bifidobacterium longum derived from Lacto-B culture in powder form. After 21 d, the rats continued to receive restricted feeding and supplemented with goat milk yoghurt for 7 d. Total splenocytes were counted by hemocytometer. Splenocytes proliferation was expressed as stimulation index, whereas the TNF-α and IL-10 of spleen lymphocyte culture were measured by ELISA technique. The total number of splenocytes and stimulation index of splenocytes in moderate malnourished and normal rats supplemented with goat milk yoghurt was not significantly different. The level of TNF-α in the rat supplemented with goat milk yoghurt was lower (p<0.05) than the control group, whereas the level of IL-10 in the rat supplemented with goat milk yoghurt was higher (p<0.05) than the control group. In conclusion, goat milk yoghurt supplementation in malnourished rats could decrease TNF-α as a representation of the proinflammatory cytokine, while it increases IL-10 as a representation of the anti-inflammatory cytokine. Korean Society for Food Science of Animal Resources 2014 2014-02-28 /pmc/articles/PMC4597830/ /pubmed/26760750 http://dx.doi.org/10.5851/kosfa.2014.34.1.88 Text en Copyright © 2014, Korean Society for Food Science of Animal Resources http://creativecommons.org/licences/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licences/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Nurliyani, Kandarina, BJ. Istiti Kusuma, Sari Trisnasari, Yunita Dewi Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title | Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title_full | Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title_fullStr | Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title_full_unstemmed | Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title_short | Goat Milk Yoghurt by Using Lacto-B Culture Modulates the Production of Tumor Necrosis Factor-Alpha and Interleukin-10 in Malnourished Rats |
title_sort | goat milk yoghurt by using lacto-b culture modulates the production of tumor necrosis factor-alpha and interleukin-10 in malnourished rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4597830/ https://www.ncbi.nlm.nih.gov/pubmed/26760750 http://dx.doi.org/10.5851/kosfa.2014.34.1.88 |
work_keys_str_mv | AT nurliyani goatmilkyoghurtbyusinglactobculturemodulatestheproductionoftumornecrosisfactoralphaandinterleukin10inmalnourishedrats AT kandarinabjistiti goatmilkyoghurtbyusinglactobculturemodulatestheproductionoftumornecrosisfactoralphaandinterleukin10inmalnourishedrats AT kusumasari goatmilkyoghurtbyusinglactobculturemodulatestheproductionoftumornecrosisfactoralphaandinterleukin10inmalnourishedrats AT trisnasariyunitadewi goatmilkyoghurtbyusinglactobculturemodulatestheproductionoftumornecrosisfactoralphaandinterleukin10inmalnourishedrats |