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A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota
Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598160/ https://www.ncbi.nlm.nih.gov/pubmed/26448569 http://dx.doi.org/10.1371/journal.pgen.1005538 |
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author | Lapidot, Moshe Karniel, Uri Gelbart, Dana Fogel, Doron Evenor, Dalia Kutsher, Yaarit Makhbash, Zion Nahon, Sahadia Shlomo, Haviva Chen, Lea Reuveni, Moshe Levin, Ilan |
author_facet | Lapidot, Moshe Karniel, Uri Gelbart, Dana Fogel, Doron Evenor, Dalia Kutsher, Yaarit Makhbash, Zion Nahon, Sahadia Shlomo, Haviva Chen, Lea Reuveni, Moshe Levin, Ilan |
author_sort | Lapidot, Moshe |
collection | PubMed |
description | Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, the association between 27 polymorphic DNA markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with TYLCV in 51 segregating recombinant populations were analyzed. These analyses localized ty-5 into a 425 bp region containing two transversions: one in the first exon of a gene encoding the tomato homolog of the messenger RNA surveillance factor Pelota (Pelo), and a second in its proximal promoter. Analyses of susceptible and resistant lines revealed that the relative transcript level of the gene remained unchanged, regardless of whether the plants were infected with TYLCV or not. This suggests that the polymorphism discovered in the coding region of the gene controls the resistance. Silencing of Pelo in a susceptible line rendered the transgenic plants highly resistant, while in the resistant line TY172 had no effect on symptom development. In addition, over-expression of the susceptible allele of the gene in the resistant TY172 line rendered it susceptible, while over-expression of the resistant allele in susceptible plants had no effect. These results confirm that Pelo is the gene controlling resistance at the ty-5 locus. Pelo, implicated in the ribosome recycling-phase of protein synthesis, offers an alternative route to promote resistance to TYLCV and other viruses. |
format | Online Article Text |
id | pubmed-4598160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45981602015-10-20 A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota Lapidot, Moshe Karniel, Uri Gelbart, Dana Fogel, Doron Evenor, Dalia Kutsher, Yaarit Makhbash, Zion Nahon, Sahadia Shlomo, Haviva Chen, Lea Reuveni, Moshe Levin, Ilan PLoS Genet Research Article Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, the association between 27 polymorphic DNA markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with TYLCV in 51 segregating recombinant populations were analyzed. These analyses localized ty-5 into a 425 bp region containing two transversions: one in the first exon of a gene encoding the tomato homolog of the messenger RNA surveillance factor Pelota (Pelo), and a second in its proximal promoter. Analyses of susceptible and resistant lines revealed that the relative transcript level of the gene remained unchanged, regardless of whether the plants were infected with TYLCV or not. This suggests that the polymorphism discovered in the coding region of the gene controls the resistance. Silencing of Pelo in a susceptible line rendered the transgenic plants highly resistant, while in the resistant line TY172 had no effect on symptom development. In addition, over-expression of the susceptible allele of the gene in the resistant TY172 line rendered it susceptible, while over-expression of the resistant allele in susceptible plants had no effect. These results confirm that Pelo is the gene controlling resistance at the ty-5 locus. Pelo, implicated in the ribosome recycling-phase of protein synthesis, offers an alternative route to promote resistance to TYLCV and other viruses. Public Library of Science 2015-10-08 /pmc/articles/PMC4598160/ /pubmed/26448569 http://dx.doi.org/10.1371/journal.pgen.1005538 Text en © 2015 Lapidot et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Lapidot, Moshe Karniel, Uri Gelbart, Dana Fogel, Doron Evenor, Dalia Kutsher, Yaarit Makhbash, Zion Nahon, Sahadia Shlomo, Haviva Chen, Lea Reuveni, Moshe Levin, Ilan A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title | A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title_full | A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title_fullStr | A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title_full_unstemmed | A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title_short | A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota |
title_sort | novel route controlling begomovirus resistance by the messenger rna surveillance factor pelota |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598160/ https://www.ncbi.nlm.nih.gov/pubmed/26448569 http://dx.doi.org/10.1371/journal.pgen.1005538 |
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