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The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membran...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598652/ https://www.ncbi.nlm.nih.gov/pubmed/26308060 http://dx.doi.org/10.3390/life5031539 |
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author | Jensen, Sara Munk Neesgaard, Vinnie Lund Skjoldbjerg, Sandra Landbo Nedergaard Brandl, Martin Ejsing, Christer S. Treusch, Alexander H. |
author_facet | Jensen, Sara Munk Neesgaard, Vinnie Lund Skjoldbjerg, Sandra Landbo Nedergaard Brandl, Martin Ejsing, Christer S. Treusch, Alexander H. |
author_sort | Jensen, Sara Munk |
collection | PubMed |
description | The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids. |
format | Online Article Text |
id | pubmed-4598652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45986522015-10-15 The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii Jensen, Sara Munk Neesgaard, Vinnie Lund Skjoldbjerg, Sandra Landbo Nedergaard Brandl, Martin Ejsing, Christer S. Treusch, Alexander H. Life (Basel) Article The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids. MDPI 2015-08-25 /pmc/articles/PMC4598652/ /pubmed/26308060 http://dx.doi.org/10.3390/life5031539 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jensen, Sara Munk Neesgaard, Vinnie Lund Skjoldbjerg, Sandra Landbo Nedergaard Brandl, Martin Ejsing, Christer S. Treusch, Alexander H. The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title_full | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title_fullStr | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title_full_unstemmed | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title_short | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
title_sort | effects of temperature and growth phase on the lipidomes of sulfolobus islandicus and sulfolobus tokodaii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598652/ https://www.ncbi.nlm.nih.gov/pubmed/26308060 http://dx.doi.org/10.3390/life5031539 |
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