Cargando…

The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii

The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membran...

Descripción completa

Detalles Bibliográficos
Autores principales: Jensen, Sara Munk, Neesgaard, Vinnie Lund, Skjoldbjerg, Sandra Landbo Nedergaard, Brandl, Martin, Ejsing, Christer S., Treusch, Alexander H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598652/
https://www.ncbi.nlm.nih.gov/pubmed/26308060
http://dx.doi.org/10.3390/life5031539
_version_ 1782394101979676672
author Jensen, Sara Munk
Neesgaard, Vinnie Lund
Skjoldbjerg, Sandra Landbo Nedergaard
Brandl, Martin
Ejsing, Christer S.
Treusch, Alexander H.
author_facet Jensen, Sara Munk
Neesgaard, Vinnie Lund
Skjoldbjerg, Sandra Landbo Nedergaard
Brandl, Martin
Ejsing, Christer S.
Treusch, Alexander H.
author_sort Jensen, Sara Munk
collection PubMed
description The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids.
format Online
Article
Text
id pubmed-4598652
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45986522015-10-15 The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii Jensen, Sara Munk Neesgaard, Vinnie Lund Skjoldbjerg, Sandra Landbo Nedergaard Brandl, Martin Ejsing, Christer S. Treusch, Alexander H. Life (Basel) Article The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids. MDPI 2015-08-25 /pmc/articles/PMC4598652/ /pubmed/26308060 http://dx.doi.org/10.3390/life5031539 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jensen, Sara Munk
Neesgaard, Vinnie Lund
Skjoldbjerg, Sandra Landbo Nedergaard
Brandl, Martin
Ejsing, Christer S.
Treusch, Alexander H.
The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title_full The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title_fullStr The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title_full_unstemmed The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title_short The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
title_sort effects of temperature and growth phase on the lipidomes of sulfolobus islandicus and sulfolobus tokodaii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598652/
https://www.ncbi.nlm.nih.gov/pubmed/26308060
http://dx.doi.org/10.3390/life5031539
work_keys_str_mv AT jensensaramunk theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT neesgaardvinnielund theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT skjoldbjergsandralandbonedergaard theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT brandlmartin theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT ejsingchristers theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT treuschalexanderh theeffectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT jensensaramunk effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT neesgaardvinnielund effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT skjoldbjergsandralandbonedergaard effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT brandlmartin effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT ejsingchristers effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii
AT treuschalexanderh effectsoftemperatureandgrowthphaseonthelipidomesofsulfolobusislandicusandsulfolobustokodaii