Cargando…

Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia

Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified in Aedes aegypti and some have been associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if m...

Descripción completa

Detalles Bibliográficos
Autores principales: Rochmijati Wuliandari, Juli, Lee, Siu Fai, White, Vanessa Linley, Tantowijoyo, Warsito, Hoffmann, Ary Anthony, Endersby-Harshman, Nancy Margaret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598657/
https://www.ncbi.nlm.nih.gov/pubmed/26463408
http://dx.doi.org/10.3390/insects6030658
_version_ 1782394103102701568
author Rochmijati Wuliandari, Juli
Lee, Siu Fai
White, Vanessa Linley
Tantowijoyo, Warsito
Hoffmann, Ary Anthony
Endersby-Harshman, Nancy Margaret
author_facet Rochmijati Wuliandari, Juli
Lee, Siu Fai
White, Vanessa Linley
Tantowijoyo, Warsito
Hoffmann, Ary Anthony
Endersby-Harshman, Nancy Margaret
author_sort Rochmijati Wuliandari, Juli
collection PubMed
description Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified in Aedes aegypti and some have been associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if mutations are to become markers for resistance monitoring. We describe High Resolution Melt (HRM) genotyping assays for assessing mutations found in Ae. aegypti in Indonesia (F1565C, V1023G, S996P) and use them to test for associations with pyrethroid resistance in mosquitoes from Yogyakarta, a city where insecticide use is widespread. Such knowledge is important because Yogyakarta is a target area for releases of Wolbachia-infected mosquitoes with virus-blocking traits for dengue suppression. We identify three alleles across Yogyakarta putatively linked to resistance in previous research. By comparing resistant and susceptible mosquitoes from bioassays, we show that the 1023G allele is associated with resistance to type I and type II pyrethroids. In contrast, F1565C homozygotes were rare and there was only a weak association between individuals heterozygous for the mutation and resistance to a type I pyrethroid. As the heterozygote is expected to be incompletely recessive, it is likely that this association was due to a different resistance mechanism being present. A resistance advantage conferred to V1023G homozygotes through addition of the S996P allele in the homozygous form was suggested for the Type II pyrethroid, deltamethrin. Screening of V1023G and S996P should assist resistance monitoring in Ae. aegypti from Yogyakarta, and these mutations should be maintained in Wolbachia strains destined for release in this city to ensure that these virus-blocking strains of mosquitoes are not disadvantaged, relative to resident populations.
format Online
Article
Text
id pubmed-4598657
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45986572015-10-15 Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia Rochmijati Wuliandari, Juli Lee, Siu Fai White, Vanessa Linley Tantowijoyo, Warsito Hoffmann, Ary Anthony Endersby-Harshman, Nancy Margaret Insects Article Mutations in the voltage-sensitive sodium channel gene (Vssc) have been identified in Aedes aegypti and some have been associated with pyrethroid insecticide resistance. Whether these mutations cause resistance, alone or in combination with other alleles, remains unclear, but must be understood if mutations are to become markers for resistance monitoring. We describe High Resolution Melt (HRM) genotyping assays for assessing mutations found in Ae. aegypti in Indonesia (F1565C, V1023G, S996P) and use them to test for associations with pyrethroid resistance in mosquitoes from Yogyakarta, a city where insecticide use is widespread. Such knowledge is important because Yogyakarta is a target area for releases of Wolbachia-infected mosquitoes with virus-blocking traits for dengue suppression. We identify three alleles across Yogyakarta putatively linked to resistance in previous research. By comparing resistant and susceptible mosquitoes from bioassays, we show that the 1023G allele is associated with resistance to type I and type II pyrethroids. In contrast, F1565C homozygotes were rare and there was only a weak association between individuals heterozygous for the mutation and resistance to a type I pyrethroid. As the heterozygote is expected to be incompletely recessive, it is likely that this association was due to a different resistance mechanism being present. A resistance advantage conferred to V1023G homozygotes through addition of the S996P allele in the homozygous form was suggested for the Type II pyrethroid, deltamethrin. Screening of V1023G and S996P should assist resistance monitoring in Ae. aegypti from Yogyakarta, and these mutations should be maintained in Wolbachia strains destined for release in this city to ensure that these virus-blocking strains of mosquitoes are not disadvantaged, relative to resident populations. MDPI 2015-07-23 /pmc/articles/PMC4598657/ /pubmed/26463408 http://dx.doi.org/10.3390/insects6030658 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rochmijati Wuliandari, Juli
Lee, Siu Fai
White, Vanessa Linley
Tantowijoyo, Warsito
Hoffmann, Ary Anthony
Endersby-Harshman, Nancy Margaret
Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title_full Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title_fullStr Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title_full_unstemmed Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title_short Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia
title_sort association between three mutations, f1565c, v1023g and s996p, in the voltage-sensitive sodium channel gene and knockdown resistance in aedes aegypti from yogyakarta, indonesia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598657/
https://www.ncbi.nlm.nih.gov/pubmed/26463408
http://dx.doi.org/10.3390/insects6030658
work_keys_str_mv AT rochmijatiwuliandarijuli associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia
AT leesiufai associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia
AT whitevanessalinley associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia
AT tantowijoyowarsito associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia
AT hoffmannaryanthony associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia
AT endersbyharshmannancymargaret associationbetweenthreemutationsf1565cv1023gands996pinthevoltagesensitivesodiumchannelgeneandknockdownresistanceinaedesaegyptifromyogyakartaindonesia