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Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications

Tubular scaffolds with aligned polylactic acid (PLA) fibres were fabricated for cell guide applications by immersing rolled PLA fibre mats into a polyvinyl acetate (PVAc) solution to bind the mats. The PVAc solution was also mixed with up to 30 wt % β-tricalcium phosphate (β-TCP) content. Cross-sect...

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Autores principales: Hossain, Kazi M. Zakir, Zhu, Chenkai, Felfel, Reda M., Sharmin, Nusrat, Ahmed, Ifty
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598671/
https://www.ncbi.nlm.nih.gov/pubmed/26184328
http://dx.doi.org/10.3390/jfb6030564
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author Hossain, Kazi M. Zakir
Zhu, Chenkai
Felfel, Reda M.
Sharmin, Nusrat
Ahmed, Ifty
author_facet Hossain, Kazi M. Zakir
Zhu, Chenkai
Felfel, Reda M.
Sharmin, Nusrat
Ahmed, Ifty
author_sort Hossain, Kazi M. Zakir
collection PubMed
description Tubular scaffolds with aligned polylactic acid (PLA) fibres were fabricated for cell guide applications by immersing rolled PLA fibre mats into a polyvinyl acetate (PVAc) solution to bind the mats. The PVAc solution was also mixed with up to 30 wt % β-tricalcium phosphate (β-TCP) content. Cross-sectional images of the scaffold materials obtained via scanning electron microscopy (SEM) revealed the aligned fibre morphology along with a significant number of voids in between the bundles of fibres. The addition of β-TCP into the scaffolds played an important role in increasing the void content from 17.1% to 25.3% for the 30 wt % β-TCP loading, which was measured via micro-CT (µCT) analysis. Furthermore, µCT analyses revealed the distribution of aggregated β-TCP particles in between the various PLA fibre layers of the scaffold. The compressive modulus properties of the scaffolds increased from 66 MPa to 83 MPa and the compressive strength properties decreased from 67 MPa to 41 MPa for the 30 wt % β-TCP content scaffold. The scaffolds produced were observed to change into a soft and flexible form which demonstrated shape recovery properties after immersion in phosphate buffered saline (PBS) media at 37 °C for 24 h. The cytocompatibility studies (using MG-63 human osteosarcoma cell line) revealed preferential cell proliferation along the longitudinal direction of the fibres as compared to the control tissue culture plastic. The manufacturing process highlighted above reveals a simple process for inducing controlled cell alignment and varying porosity features within tubular scaffolds for potential tissue engineering applications.
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spelling pubmed-45986712015-10-15 Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications Hossain, Kazi M. Zakir Zhu, Chenkai Felfel, Reda M. Sharmin, Nusrat Ahmed, Ifty J Funct Biomater Article Tubular scaffolds with aligned polylactic acid (PLA) fibres were fabricated for cell guide applications by immersing rolled PLA fibre mats into a polyvinyl acetate (PVAc) solution to bind the mats. The PVAc solution was also mixed with up to 30 wt % β-tricalcium phosphate (β-TCP) content. Cross-sectional images of the scaffold materials obtained via scanning electron microscopy (SEM) revealed the aligned fibre morphology along with a significant number of voids in between the bundles of fibres. The addition of β-TCP into the scaffolds played an important role in increasing the void content from 17.1% to 25.3% for the 30 wt % β-TCP loading, which was measured via micro-CT (µCT) analysis. Furthermore, µCT analyses revealed the distribution of aggregated β-TCP particles in between the various PLA fibre layers of the scaffold. The compressive modulus properties of the scaffolds increased from 66 MPa to 83 MPa and the compressive strength properties decreased from 67 MPa to 41 MPa for the 30 wt % β-TCP content scaffold. The scaffolds produced were observed to change into a soft and flexible form which demonstrated shape recovery properties after immersion in phosphate buffered saline (PBS) media at 37 °C for 24 h. The cytocompatibility studies (using MG-63 human osteosarcoma cell line) revealed preferential cell proliferation along the longitudinal direction of the fibres as compared to the control tissue culture plastic. The manufacturing process highlighted above reveals a simple process for inducing controlled cell alignment and varying porosity features within tubular scaffolds for potential tissue engineering applications. MDPI 2015-07-10 /pmc/articles/PMC4598671/ /pubmed/26184328 http://dx.doi.org/10.3390/jfb6030564 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hossain, Kazi M. Zakir
Zhu, Chenkai
Felfel, Reda M.
Sharmin, Nusrat
Ahmed, Ifty
Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title_full Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title_fullStr Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title_full_unstemmed Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title_short Tubular Scaffold with Shape Recovery Effect for Cell Guide Applications
title_sort tubular scaffold with shape recovery effect for cell guide applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598671/
https://www.ncbi.nlm.nih.gov/pubmed/26184328
http://dx.doi.org/10.3390/jfb6030564
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