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Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications

Injectable hydrogels are gaining popularity as tissue engineering constructs because of their ease of handling and minimal invasive delivery. Making hydrogels from natural polymers helps to overcome biocompatibility issues. Here, we have developed an Amorphous Chitin (ACh)-Agarose (Agr) composite hy...

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Autores principales: Priya, Murali Vishnu, Kumar, Rajendran Arun, Sivashanmugam, Amirthalingam, Nair, Shantikumar Vasudevan, Jayakumar, Rangasamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598681/
https://www.ncbi.nlm.nih.gov/pubmed/26308065
http://dx.doi.org/10.3390/jfb6030849
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author Priya, Murali Vishnu
Kumar, Rajendran Arun
Sivashanmugam, Amirthalingam
Nair, Shantikumar Vasudevan
Jayakumar, Rangasamy
author_facet Priya, Murali Vishnu
Kumar, Rajendran Arun
Sivashanmugam, Amirthalingam
Nair, Shantikumar Vasudevan
Jayakumar, Rangasamy
author_sort Priya, Murali Vishnu
collection PubMed
description Injectable hydrogels are gaining popularity as tissue engineering constructs because of their ease of handling and minimal invasive delivery. Making hydrogels from natural polymers helps to overcome biocompatibility issues. Here, we have developed an Amorphous Chitin (ACh)-Agarose (Agr) composite hydrogel using a simpletechnique. Rheological studies, such as viscoelastic behavior (elastic modulus, viscous modulus, yield stress, and consistency), inversion test, and injectability test, were carried out for different ACh-Agr concentrations. The composite gel, having a concentration of 1.5% ACh and 0.25% Agr, showed good elastic modulus (17.3 kPa), yield stress (3.8 kPa), no flow under gravity, injectability, and temperature stability within the physiological range. Based on these studies, the optimum concentration for injectability was found to be 1.5% ACh and 0.25% Agr. This optimized concentration was used for further studies and characterized using FT-IR and SEM. FT-IR studies confirmed the presence of ACh and Agr in the composite gel. SEM results showed that the lyophilized composite gel had good porosity and mesh like networks. The cytocompatibility of the composite gel was studied using human mesenchymal stem cells (hMSCs). The composite gels showed good cell viability.These results indicated that this injectable composite gel can be used for biomedical applications.
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spelling pubmed-45986812015-10-15 Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications Priya, Murali Vishnu Kumar, Rajendran Arun Sivashanmugam, Amirthalingam Nair, Shantikumar Vasudevan Jayakumar, Rangasamy J Funct Biomater Article Injectable hydrogels are gaining popularity as tissue engineering constructs because of their ease of handling and minimal invasive delivery. Making hydrogels from natural polymers helps to overcome biocompatibility issues. Here, we have developed an Amorphous Chitin (ACh)-Agarose (Agr) composite hydrogel using a simpletechnique. Rheological studies, such as viscoelastic behavior (elastic modulus, viscous modulus, yield stress, and consistency), inversion test, and injectability test, were carried out for different ACh-Agr concentrations. The composite gel, having a concentration of 1.5% ACh and 0.25% Agr, showed good elastic modulus (17.3 kPa), yield stress (3.8 kPa), no flow under gravity, injectability, and temperature stability within the physiological range. Based on these studies, the optimum concentration for injectability was found to be 1.5% ACh and 0.25% Agr. This optimized concentration was used for further studies and characterized using FT-IR and SEM. FT-IR studies confirmed the presence of ACh and Agr in the composite gel. SEM results showed that the lyophilized composite gel had good porosity and mesh like networks. The cytocompatibility of the composite gel was studied using human mesenchymal stem cells (hMSCs). The composite gels showed good cell viability.These results indicated that this injectable composite gel can be used for biomedical applications. MDPI 2015-08-25 /pmc/articles/PMC4598681/ /pubmed/26308065 http://dx.doi.org/10.3390/jfb6030849 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Priya, Murali Vishnu
Kumar, Rajendran Arun
Sivashanmugam, Amirthalingam
Nair, Shantikumar Vasudevan
Jayakumar, Rangasamy
Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title_full Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title_fullStr Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title_full_unstemmed Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title_short Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications
title_sort injectable amorphous chitin-agarose composite hydrogels for biomedical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598681/
https://www.ncbi.nlm.nih.gov/pubmed/26308065
http://dx.doi.org/10.3390/jfb6030849
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