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Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk

The outstanding combination of high tensile strength and extensibility of spider silk is believed to contribute to the material’s toughness. Thus, there is great interest in engineering silk for biomedical products such as suture or implants. Additionally, over the years, many studies have also soug...

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Autores principales: Lai, Wee Loong, Goh, Kheng Lim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598684/
https://www.ncbi.nlm.nih.gov/pubmed/26378587
http://dx.doi.org/10.3390/jfb6030901
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author Lai, Wee Loong
Goh, Kheng Lim
author_facet Lai, Wee Loong
Goh, Kheng Lim
author_sort Lai, Wee Loong
collection PubMed
description The outstanding combination of high tensile strength and extensibility of spider silk is believed to contribute to the material’s toughness. Thus, there is great interest in engineering silk for biomedical products such as suture or implants. Additionally, over the years, many studies have also sought to enhance the mechanical properties of spider silk for wider applicability, e.g., by irradiating the material using ultra-violet radiation. However, the limitations surrounding the use of ultra-violet radiation for enhancing the mechanical properties of spider silk are not well-understood. Here, we have analyzed the mechanical properties of spider silk at short ultra-violet irradiation duration. Specimens of spider silk were subjected to ultra-violet irradiation (254-nm wavelength, i.e. UVC) for 10, 20, and 30 min, respectively, followed by tensile test to rupture to determine the strength (maximum stress), extensibility (rupture strain), and toughness (strain energy density to rupture). Controls, i.e., specimens that did not received UVC, were also subjected to tensile test to rupture to determine the respective mechanical properties. One-way analysis of variance reveals that these properties decrease significantly (p < 0.05) with increasing irradiation duration. Among the three mechanical parameters, the strength of the spider silk degrades most rapidly; the extensibility of the spider silk degrades the slowest. Overall, these changes correspond to the observed surface modifications as well as the bond rupture between the peptide chains of the treated silk. Altogether, this simple but comprehensive study provides some key insights into the dependence of the mechanical properties on ultra-violet irradiation duration.
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spelling pubmed-45986842015-10-15 Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk Lai, Wee Loong Goh, Kheng Lim J Funct Biomater Article The outstanding combination of high tensile strength and extensibility of spider silk is believed to contribute to the material’s toughness. Thus, there is great interest in engineering silk for biomedical products such as suture or implants. Additionally, over the years, many studies have also sought to enhance the mechanical properties of spider silk for wider applicability, e.g., by irradiating the material using ultra-violet radiation. However, the limitations surrounding the use of ultra-violet radiation for enhancing the mechanical properties of spider silk are not well-understood. Here, we have analyzed the mechanical properties of spider silk at short ultra-violet irradiation duration. Specimens of spider silk were subjected to ultra-violet irradiation (254-nm wavelength, i.e. UVC) for 10, 20, and 30 min, respectively, followed by tensile test to rupture to determine the strength (maximum stress), extensibility (rupture strain), and toughness (strain energy density to rupture). Controls, i.e., specimens that did not received UVC, were also subjected to tensile test to rupture to determine the respective mechanical properties. One-way analysis of variance reveals that these properties decrease significantly (p < 0.05) with increasing irradiation duration. Among the three mechanical parameters, the strength of the spider silk degrades most rapidly; the extensibility of the spider silk degrades the slowest. Overall, these changes correspond to the observed surface modifications as well as the bond rupture between the peptide chains of the treated silk. Altogether, this simple but comprehensive study provides some key insights into the dependence of the mechanical properties on ultra-violet irradiation duration. MDPI 2015-09-10 /pmc/articles/PMC4598684/ /pubmed/26378587 http://dx.doi.org/10.3390/jfb6030901 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lai, Wee Loong
Goh, Kheng Lim
Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title_full Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title_fullStr Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title_full_unstemmed Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title_short Consequences of Ultra-Violet Irradiation on the Mechanical Properties of Spider Silk
title_sort consequences of ultra-violet irradiation on the mechanical properties of spider silk
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598684/
https://www.ncbi.nlm.nih.gov/pubmed/26378587
http://dx.doi.org/10.3390/jfb6030901
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