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Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche
Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and f...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598714/ https://www.ncbi.nlm.nih.gov/pubmed/26452202 http://dx.doi.org/10.7554/eLife.08174 |
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author | Wang, Su Gao, Yuan Song, Xiaoqing Ma, Xing Zhu, Xiujuan Mao, Ying Yang, Zhihao Ni, Jianquan Li, Hua Malanowski, Kathryn E Anoja, Perera Park, Jungeun Haug, Jeff Xie, Ting |
author_facet | Wang, Su Gao, Yuan Song, Xiaoqing Ma, Xing Zhu, Xiujuan Mao, Ying Yang, Zhihao Ni, Jianquan Li, Hua Malanowski, Kathryn E Anoja, Perera Park, Jungeun Haug, Jeff Xie, Ting |
author_sort | Wang, Su |
collection | PubMed |
description | Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. In this study, we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote GSC progeny differentiation. Defective Wnt signaling causes the loss of the differentiation niche and the upregulated BMP signaling in differentiated GSC progeny, thereby disrupting germ cell differentiation. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche. DOI: http://dx.doi.org/10.7554/eLife.08174.001 |
format | Online Article Text |
id | pubmed-4598714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45987142015-10-10 Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche Wang, Su Gao, Yuan Song, Xiaoqing Ma, Xing Zhu, Xiujuan Mao, Ying Yang, Zhihao Ni, Jianquan Li, Hua Malanowski, Kathryn E Anoja, Perera Park, Jungeun Haug, Jeff Xie, Ting eLife Developmental Biology and Stem Cells Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. In this study, we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote GSC progeny differentiation. Defective Wnt signaling causes the loss of the differentiation niche and the upregulated BMP signaling in differentiated GSC progeny, thereby disrupting germ cell differentiation. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche. DOI: http://dx.doi.org/10.7554/eLife.08174.001 eLife Sciences Publications, Ltd 2015-10-09 /pmc/articles/PMC4598714/ /pubmed/26452202 http://dx.doi.org/10.7554/eLife.08174 Text en © 2015, Wang et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Wang, Su Gao, Yuan Song, Xiaoqing Ma, Xing Zhu, Xiujuan Mao, Ying Yang, Zhihao Ni, Jianquan Li, Hua Malanowski, Kathryn E Anoja, Perera Park, Jungeun Haug, Jeff Xie, Ting Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title | Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title_full | Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title_fullStr | Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title_full_unstemmed | Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title_short | Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
title_sort | wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598714/ https://www.ncbi.nlm.nih.gov/pubmed/26452202 http://dx.doi.org/10.7554/eLife.08174 |
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