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High-Resolution Respirometry for Simultaneous Measurement of Oxygen and Hydrogen Peroxide Fluxes in Permeabilized Cells, Tissue Homogenate and Isolated Mitochondria

Whereas mitochondria are well established as the source of ATP in oxidative phosphorylation (OXPHOS), it is debated if they are also the major cellular sources of reactive oxygen species (ROS). Here we describe the novel approach of combining high-resolution respirometry and fluorometric measurement...

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Detalles Bibliográficos
Autores principales: Makrecka-Kuka, Marina, Krumschnabel, Gerhard, Gnaiger, Erich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598754/
https://www.ncbi.nlm.nih.gov/pubmed/26131977
http://dx.doi.org/10.3390/biom5031319