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Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation
Understanding how metabolite levels change over the 24 hour day is of crucial importance for clinical and epidemiological studies. Additionally, the association between sleep deprivation and metabolic disorders such as diabetes and obesity requires investigation into the links between sleep and meta...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598809/ https://www.ncbi.nlm.nih.gov/pubmed/26450397 http://dx.doi.org/10.1038/srep14843 |
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author | Giskeødegård, Guro F. Davies, Sarah K. Revell, Victoria L. Keun, Hector Skene, Debra J. |
author_facet | Giskeødegård, Guro F. Davies, Sarah K. Revell, Victoria L. Keun, Hector Skene, Debra J. |
author_sort | Giskeødegård, Guro F. |
collection | PubMed |
description | Understanding how metabolite levels change over the 24 hour day is of crucial importance for clinical and epidemiological studies. Additionally, the association between sleep deprivation and metabolic disorders such as diabetes and obesity requires investigation into the links between sleep and metabolism. Here, we characterise time-of-day variation and the effects of sleep deprivation on urinary metabolite profiles. Healthy male participants (n = 15) completed an in-laboratory study comprising one 24 h sleep/wake cycle prior to 24 h of continual wakefulness under highly controlled environmental conditions. Urine samples were collected over set 2–8 h intervals and analysed by (1)H NMR spectroscopy. Significant changes were observed with respect to both time of day and sleep deprivation. Of 32 identified metabolites, 7 (22%) exhibited cosine rhythmicity over at least one 24 h period; 5 exhibiting a cosine rhythm on both days. Eight metabolites significantly increased during sleep deprivation compared with sleep (taurine, formate, citrate, 3-indoxyl sulfate, carnitine, 3-hydroxyisobutyrate, TMAO and acetate) and 8 significantly decreased (dimethylamine, 4-DTA, creatinine, ascorbate, 2-hydroxyisobutyrate, allantoin, 4-DEA, 4-hydroxyphenylacetate). These data indicate that sampling time, the presence or absence of sleep and the response to sleep deprivation are highly relevant when identifying biomarkers in urinary metabolic profiling studies. |
format | Online Article Text |
id | pubmed-4598809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45988092015-10-13 Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation Giskeødegård, Guro F. Davies, Sarah K. Revell, Victoria L. Keun, Hector Skene, Debra J. Sci Rep Article Understanding how metabolite levels change over the 24 hour day is of crucial importance for clinical and epidemiological studies. Additionally, the association between sleep deprivation and metabolic disorders such as diabetes and obesity requires investigation into the links between sleep and metabolism. Here, we characterise time-of-day variation and the effects of sleep deprivation on urinary metabolite profiles. Healthy male participants (n = 15) completed an in-laboratory study comprising one 24 h sleep/wake cycle prior to 24 h of continual wakefulness under highly controlled environmental conditions. Urine samples were collected over set 2–8 h intervals and analysed by (1)H NMR spectroscopy. Significant changes were observed with respect to both time of day and sleep deprivation. Of 32 identified metabolites, 7 (22%) exhibited cosine rhythmicity over at least one 24 h period; 5 exhibiting a cosine rhythm on both days. Eight metabolites significantly increased during sleep deprivation compared with sleep (taurine, formate, citrate, 3-indoxyl sulfate, carnitine, 3-hydroxyisobutyrate, TMAO and acetate) and 8 significantly decreased (dimethylamine, 4-DTA, creatinine, ascorbate, 2-hydroxyisobutyrate, allantoin, 4-DEA, 4-hydroxyphenylacetate). These data indicate that sampling time, the presence or absence of sleep and the response to sleep deprivation are highly relevant when identifying biomarkers in urinary metabolic profiling studies. Nature Publishing Group 2015-10-09 /pmc/articles/PMC4598809/ /pubmed/26450397 http://dx.doi.org/10.1038/srep14843 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Giskeødegård, Guro F. Davies, Sarah K. Revell, Victoria L. Keun, Hector Skene, Debra J. Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title | Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title_full | Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title_fullStr | Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title_full_unstemmed | Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title_short | Diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
title_sort | diurnal rhythms in the human urine metabolome during sleep and total sleep deprivation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4598809/ https://www.ncbi.nlm.nih.gov/pubmed/26450397 http://dx.doi.org/10.1038/srep14843 |
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