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PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest

Growing evidence indicates that dys-regulation of PBRM1 contributes to tumorigenesis. However, little is known about the biological function of PBRM1 in the development or progression of bladder cancer. In this study, we aimed to elucidate the pathophysiological role of PBRM1 in bladder cancer. We a...

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Autores principales: Huang, Li, Peng, Yang, Zhong, Guangzheng, Xie, Weibin, Dong, Wen, Wang, Bo, Chen, Xu, Gu, Peng, He, Wang, Wu, Shaoxu, Lin, Tianxin, Huang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599275/
https://www.ncbi.nlm.nih.gov/pubmed/25978027
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author Huang, Li
Peng, Yang
Zhong, Guangzheng
Xie, Weibin
Dong, Wen
Wang, Bo
Chen, Xu
Gu, Peng
He, Wang
Wu, Shaoxu
Lin, Tianxin
Huang, Jian
author_facet Huang, Li
Peng, Yang
Zhong, Guangzheng
Xie, Weibin
Dong, Wen
Wang, Bo
Chen, Xu
Gu, Peng
He, Wang
Wu, Shaoxu
Lin, Tianxin
Huang, Jian
author_sort Huang, Li
collection PubMed
description Growing evidence indicates that dys-regulation of PBRM1 contributes to tumorigenesis. However, little is known about the biological function of PBRM1 in the development or progression of bladder cancer. In this study, we aimed to elucidate the pathophysiological role of PBRM1 in bladder cancer. We assessed the expression of PBRM1 in 64 bladder cancer tissue samples with matching normal tissues. We explored the biological functions of PBRM1 both in vitro and in vivo. Mutational status of PBRM1 was analyzed. Effect of PBRM1 on cell cycle was evaluated. qRT-PCR and Western blot were carried out to evaluate the expression of cyclins affected by PBRM1. Our results showed that PBRM1 expression was significantly reduced in bladder cancer cells and tissues compared to their normal counterparts. The reduced expression of PBRM1 was associated with advanced tumor stage, low differentiation grade and worse patient outcome. Further functional analysis demonstrated that PBRM1 suppressed bladder cancer cell proliferation, migration, colony formation in vitro and tumorigenicity in vivo. Genetic alteration analysis showed no amino-acid sequence altering mutations. We found that PBRM1 could block the G2/M transition by repressing cyclin B1. Our data indicated that PBRM1 functions as a tumor suppressor in bladder cancer by repressing cyclin B1 expression.
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spelling pubmed-45992752015-10-26 PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest Huang, Li Peng, Yang Zhong, Guangzheng Xie, Weibin Dong, Wen Wang, Bo Chen, Xu Gu, Peng He, Wang Wu, Shaoxu Lin, Tianxin Huang, Jian Oncotarget Research Paper Growing evidence indicates that dys-regulation of PBRM1 contributes to tumorigenesis. However, little is known about the biological function of PBRM1 in the development or progression of bladder cancer. In this study, we aimed to elucidate the pathophysiological role of PBRM1 in bladder cancer. We assessed the expression of PBRM1 in 64 bladder cancer tissue samples with matching normal tissues. We explored the biological functions of PBRM1 both in vitro and in vivo. Mutational status of PBRM1 was analyzed. Effect of PBRM1 on cell cycle was evaluated. qRT-PCR and Western blot were carried out to evaluate the expression of cyclins affected by PBRM1. Our results showed that PBRM1 expression was significantly reduced in bladder cancer cells and tissues compared to their normal counterparts. The reduced expression of PBRM1 was associated with advanced tumor stage, low differentiation grade and worse patient outcome. Further functional analysis demonstrated that PBRM1 suppressed bladder cancer cell proliferation, migration, colony formation in vitro and tumorigenicity in vivo. Genetic alteration analysis showed no amino-acid sequence altering mutations. We found that PBRM1 could block the G2/M transition by repressing cyclin B1. Our data indicated that PBRM1 functions as a tumor suppressor in bladder cancer by repressing cyclin B1 expression. Impact Journals LLC 2015-04-19 /pmc/articles/PMC4599275/ /pubmed/25978027 Text en Copyright: © 2015 Huang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Huang, Li
Peng, Yang
Zhong, Guangzheng
Xie, Weibin
Dong, Wen
Wang, Bo
Chen, Xu
Gu, Peng
He, Wang
Wu, Shaoxu
Lin, Tianxin
Huang, Jian
PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title_full PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title_fullStr PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title_full_unstemmed PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title_short PBRM1 suppresses bladder cancer by cyclin B1 induced cell cycle arrest
title_sort pbrm1 suppresses bladder cancer by cyclin b1 induced cell cycle arrest
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599275/
https://www.ncbi.nlm.nih.gov/pubmed/25978027
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