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Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

The severe inflammation observed during sepsis is thought to cause diaphragm dysfunction, which is associated with poor patient prognosis. Cerium oxide (CeO(2)) nanoparticles have been posited to exhibit anti-inflammatory and antioxidative activities suggesting that these particles may be of potenti...

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Autores principales: Asano, Shinichi, Arvapalli, Ravikumar, Manne, Nandini DPK, Maheshwari, Mani, Ma, Bing, Rice, Kevin M, Selvaraj, Vellaisamy, Blough, Eric R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599716/
https://www.ncbi.nlm.nih.gov/pubmed/26491293
http://dx.doi.org/10.2147/IJN.S89783
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author Asano, Shinichi
Arvapalli, Ravikumar
Manne, Nandini DPK
Maheshwari, Mani
Ma, Bing
Rice, Kevin M
Selvaraj, Vellaisamy
Blough, Eric R
author_facet Asano, Shinichi
Arvapalli, Ravikumar
Manne, Nandini DPK
Maheshwari, Mani
Ma, Bing
Rice, Kevin M
Selvaraj, Vellaisamy
Blough, Eric R
author_sort Asano, Shinichi
collection PubMed
description The severe inflammation observed during sepsis is thought to cause diaphragm dysfunction, which is associated with poor patient prognosis. Cerium oxide (CeO(2)) nanoparticles have been posited to exhibit anti-inflammatory and antioxidative activities suggesting that these particles may be of potential use for the treatment of inflammatory disorders. To investigate this possibility, Sprague Dawley rats were randomly assigned to the following groups: sham control, CeO(2) nanoparticle treatment only (0.5 mg/kg iv), sepsis, and sepsis+CeO(2) nanoparticles. Sepsis was induced by the introduction of cecal material (600 mg/kg) directly into the peritoneal cavity. Nanoparticle treatment decreased sepsis-associated impairments in diaphragmatic contractile (P(o)) function (sham: 25.6±1.6 N/cm(2) vs CeO(2): 23.4±0.8 N/cm(2) vs Sep: 15.9±1.0 N/cm(2) vs Sep+CeO(2): 20.0±1.0 N/cm(2), P<0.05). These improvements in diaphragm contractile function were accompanied by a normalization of protein translation signaling (Akt, FOXO-1, and 4EBP1), diminished proteolysis (caspase 8 and ubiquitin levels), and decreased inflammatory signaling (Stat3 and iNOS). Histological analysis suggested that nanoparticle treatment was associated with diminished sarcolemma damage and diminished inflammatory cell infiltration. These data indicate CeO(2) nanoparticles may improve diaphragmatic function in the septic laboratory rat.
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spelling pubmed-45997162015-10-21 Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction Asano, Shinichi Arvapalli, Ravikumar Manne, Nandini DPK Maheshwari, Mani Ma, Bing Rice, Kevin M Selvaraj, Vellaisamy Blough, Eric R Int J Nanomedicine Original Research The severe inflammation observed during sepsis is thought to cause diaphragm dysfunction, which is associated with poor patient prognosis. Cerium oxide (CeO(2)) nanoparticles have been posited to exhibit anti-inflammatory and antioxidative activities suggesting that these particles may be of potential use for the treatment of inflammatory disorders. To investigate this possibility, Sprague Dawley rats were randomly assigned to the following groups: sham control, CeO(2) nanoparticle treatment only (0.5 mg/kg iv), sepsis, and sepsis+CeO(2) nanoparticles. Sepsis was induced by the introduction of cecal material (600 mg/kg) directly into the peritoneal cavity. Nanoparticle treatment decreased sepsis-associated impairments in diaphragmatic contractile (P(o)) function (sham: 25.6±1.6 N/cm(2) vs CeO(2): 23.4±0.8 N/cm(2) vs Sep: 15.9±1.0 N/cm(2) vs Sep+CeO(2): 20.0±1.0 N/cm(2), P<0.05). These improvements in diaphragm contractile function were accompanied by a normalization of protein translation signaling (Akt, FOXO-1, and 4EBP1), diminished proteolysis (caspase 8 and ubiquitin levels), and decreased inflammatory signaling (Stat3 and iNOS). Histological analysis suggested that nanoparticle treatment was associated with diminished sarcolemma damage and diminished inflammatory cell infiltration. These data indicate CeO(2) nanoparticles may improve diaphragmatic function in the septic laboratory rat. Dove Medical Press 2015-10-05 /pmc/articles/PMC4599716/ /pubmed/26491293 http://dx.doi.org/10.2147/IJN.S89783 Text en © 2015 Asano et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Asano, Shinichi
Arvapalli, Ravikumar
Manne, Nandini DPK
Maheshwari, Mani
Ma, Bing
Rice, Kevin M
Selvaraj, Vellaisamy
Blough, Eric R
Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title_full Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title_fullStr Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title_full_unstemmed Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title_short Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
title_sort cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4599716/
https://www.ncbi.nlm.nih.gov/pubmed/26491293
http://dx.doi.org/10.2147/IJN.S89783
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