Cargando…
Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers
An estimated one-third of the world’s population is currently latently infected with Mycobacterium tuberculosis. Latent M. tuberculosis infection (LTBI) progresses into active tuberculosis (TB) disease in ~5 to 10% of infected individuals. Diagnostic and prognostic biomarkers to monitor disease prog...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600108/ https://www.ncbi.nlm.nih.gov/pubmed/26374119 http://dx.doi.org/10.1128/mBio.01187-15 |
_version_ | 1782394368058982400 |
---|---|
author | Esterhuyse, Maria M. Weiner, January Caron, Etienne Loxton, Andre G. Iannaccone, Marco Wagman, Chandre Saikali, Philippe Stanley, Kim Wolski, Witold E. Mollenkopf, Hans-Joachim Schick, Matthias Aebersold, Ruedi Linhart, Heinz Walzl, Gerhard Kaufmann, Stefan H. E. |
author_facet | Esterhuyse, Maria M. Weiner, January Caron, Etienne Loxton, Andre G. Iannaccone, Marco Wagman, Chandre Saikali, Philippe Stanley, Kim Wolski, Witold E. Mollenkopf, Hans-Joachim Schick, Matthias Aebersold, Ruedi Linhart, Heinz Walzl, Gerhard Kaufmann, Stefan H. E. |
author_sort | Esterhuyse, Maria M. |
collection | PubMed |
description | An estimated one-third of the world’s population is currently latently infected with Mycobacterium tuberculosis. Latent M. tuberculosis infection (LTBI) progresses into active tuberculosis (TB) disease in ~5 to 10% of infected individuals. Diagnostic and prognostic biomarkers to monitor disease progression are urgently needed to ensure better care for TB patients and to decrease the spread of TB. Biomarker development is primarily based on transcriptomics. Our understanding of biology combined with evolving technical advances in high-throughput techniques led us to investigate the possibility of additional platforms (epigenetics and proteomics) in the quest to (i) understand the biology of the TB host response and (ii) search for multiplatform biosignatures in TB. We engaged in a pilot study to interrogate the DNA methylome, transcriptome, and proteome in selected monocytes and granulocytes from TB patients and healthy LTBI participants. Our study provides first insights into the levels and sources of diversity in the epigenome and proteome among TB patients and LTBI controls, despite limitations due to small sample size. Functionally the differences between the infection phenotypes (LTBI versus active TB) observed in the different platforms were congruent, thereby suggesting regulation of function not only at the transcriptional level but also by DNA methylation and microRNA. Thus, our data argue for the development of a large-scale study of the DNA methylome, with particular attention to study design in accounting for variation based on gender, age, and cell type. |
format | Online Article Text |
id | pubmed-4600108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46001082015-10-12 Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers Esterhuyse, Maria M. Weiner, January Caron, Etienne Loxton, Andre G. Iannaccone, Marco Wagman, Chandre Saikali, Philippe Stanley, Kim Wolski, Witold E. Mollenkopf, Hans-Joachim Schick, Matthias Aebersold, Ruedi Linhart, Heinz Walzl, Gerhard Kaufmann, Stefan H. E. mBio Research Article An estimated one-third of the world’s population is currently latently infected with Mycobacterium tuberculosis. Latent M. tuberculosis infection (LTBI) progresses into active tuberculosis (TB) disease in ~5 to 10% of infected individuals. Diagnostic and prognostic biomarkers to monitor disease progression are urgently needed to ensure better care for TB patients and to decrease the spread of TB. Biomarker development is primarily based on transcriptomics. Our understanding of biology combined with evolving technical advances in high-throughput techniques led us to investigate the possibility of additional platforms (epigenetics and proteomics) in the quest to (i) understand the biology of the TB host response and (ii) search for multiplatform biosignatures in TB. We engaged in a pilot study to interrogate the DNA methylome, transcriptome, and proteome in selected monocytes and granulocytes from TB patients and healthy LTBI participants. Our study provides first insights into the levels and sources of diversity in the epigenome and proteome among TB patients and LTBI controls, despite limitations due to small sample size. Functionally the differences between the infection phenotypes (LTBI versus active TB) observed in the different platforms were congruent, thereby suggesting regulation of function not only at the transcriptional level but also by DNA methylation and microRNA. Thus, our data argue for the development of a large-scale study of the DNA methylome, with particular attention to study design in accounting for variation based on gender, age, and cell type. American Society of Microbiology 2015-09-15 /pmc/articles/PMC4600108/ /pubmed/26374119 http://dx.doi.org/10.1128/mBio.01187-15 Text en Copyright © 2015 Esterhuyse et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Esterhuyse, Maria M. Weiner, January Caron, Etienne Loxton, Andre G. Iannaccone, Marco Wagman, Chandre Saikali, Philippe Stanley, Kim Wolski, Witold E. Mollenkopf, Hans-Joachim Schick, Matthias Aebersold, Ruedi Linhart, Heinz Walzl, Gerhard Kaufmann, Stefan H. E. Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title | Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title_full | Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title_fullStr | Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title_full_unstemmed | Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title_short | Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers |
title_sort | epigenetics and proteomics join transcriptomics in the quest for tuberculosis biomarkers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600108/ https://www.ncbi.nlm.nih.gov/pubmed/26374119 http://dx.doi.org/10.1128/mBio.01187-15 |
work_keys_str_mv | AT esterhuysemariam epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT weinerjanuary epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT caronetienne epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT loxtonandreg epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT iannacconemarco epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT wagmanchandre epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT saikaliphilippe epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT stanleykim epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT wolskiwitolde epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT mollenkopfhansjoachim epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT schickmatthias epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT aebersoldruedi epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT linhartheinz epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT walzlgerhard epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers AT kaufmannstefanhe epigeneticsandproteomicsjointranscriptomicsinthequestfortuberculosisbiomarkers |