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Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla

BACKGROUND: The study of bacteriophages continues to generate key information about microbial interactions in the environment. Many phenotypic characteristics of bacteriophages cannot be examined by sequencing alone, further highlighting the necessity for isolation and examination of phages from env...

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Autores principales: Malki, Kema, Kula, Alex, Bruder, Katherine, Sible, Emily, Hatzopoulos, Thomas, Steidel, Stephanie, Watkins, Siobhan C., Putonti, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600314/
https://www.ncbi.nlm.nih.gov/pubmed/26453042
http://dx.doi.org/10.1186/s12985-015-0395-0
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author Malki, Kema
Kula, Alex
Bruder, Katherine
Sible, Emily
Hatzopoulos, Thomas
Steidel, Stephanie
Watkins, Siobhan C.
Putonti, Catherine
author_facet Malki, Kema
Kula, Alex
Bruder, Katherine
Sible, Emily
Hatzopoulos, Thomas
Steidel, Stephanie
Watkins, Siobhan C.
Putonti, Catherine
author_sort Malki, Kema
collection PubMed
description BACKGROUND: The study of bacteriophages continues to generate key information about microbial interactions in the environment. Many phenotypic characteristics of bacteriophages cannot be examined by sequencing alone, further highlighting the necessity for isolation and examination of phages from environmental samples. While much of our current knowledge base has been generated by the study of marine phages, freshwater viruses are understudied in comparison. Our group has previously conducted metagenomics-based studies samples collected from Lake Michigan - the data presented in this study relate to four phages that were extracted from the same samples. FINDINGS: Four phages were extracted from Lake Michigan on the same bacterial host, exhibiting similar morphological characteristics as shown under transmission electron microscopy. Growth characteristics of the phages were unique to each isolate. Each phage demonstrated a host-range spanning several phyla of bacteria – to date, such a broad host-range is yet to be reported. Genomic data reveals genomes of a similar size, and close similarities between the Lake Michigan phages and the Pseudomonas phage PB1, however, the majority of annotated genes present were ORFans and little insight was offered into mechanisms for host-range. CONCLUSIONS: The phages isolated from Lake Michigan are capable of infecting several bacterial phyla, and demonstrate varied phenotypic characteristics despite similarities in host preference, and at the genomic level. We propose that such a broad host-range is likely related to the oligotrophic nature of Lake Michigan, and the competitive benefit that this characteristic may lend to phages in nature.
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spelling pubmed-46003142015-10-11 Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla Malki, Kema Kula, Alex Bruder, Katherine Sible, Emily Hatzopoulos, Thomas Steidel, Stephanie Watkins, Siobhan C. Putonti, Catherine Virol J Short Report BACKGROUND: The study of bacteriophages continues to generate key information about microbial interactions in the environment. Many phenotypic characteristics of bacteriophages cannot be examined by sequencing alone, further highlighting the necessity for isolation and examination of phages from environmental samples. While much of our current knowledge base has been generated by the study of marine phages, freshwater viruses are understudied in comparison. Our group has previously conducted metagenomics-based studies samples collected from Lake Michigan - the data presented in this study relate to four phages that were extracted from the same samples. FINDINGS: Four phages were extracted from Lake Michigan on the same bacterial host, exhibiting similar morphological characteristics as shown under transmission electron microscopy. Growth characteristics of the phages were unique to each isolate. Each phage demonstrated a host-range spanning several phyla of bacteria – to date, such a broad host-range is yet to be reported. Genomic data reveals genomes of a similar size, and close similarities between the Lake Michigan phages and the Pseudomonas phage PB1, however, the majority of annotated genes present were ORFans and little insight was offered into mechanisms for host-range. CONCLUSIONS: The phages isolated from Lake Michigan are capable of infecting several bacterial phyla, and demonstrate varied phenotypic characteristics despite similarities in host preference, and at the genomic level. We propose that such a broad host-range is likely related to the oligotrophic nature of Lake Michigan, and the competitive benefit that this characteristic may lend to phages in nature. BioMed Central 2015-10-09 /pmc/articles/PMC4600314/ /pubmed/26453042 http://dx.doi.org/10.1186/s12985-015-0395-0 Text en © Malki et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Malki, Kema
Kula, Alex
Bruder, Katherine
Sible, Emily
Hatzopoulos, Thomas
Steidel, Stephanie
Watkins, Siobhan C.
Putonti, Catherine
Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title_full Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title_fullStr Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title_full_unstemmed Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title_short Bacteriophages isolated from Lake Michigan demonstrate broad host-range across several bacterial phyla
title_sort bacteriophages isolated from lake michigan demonstrate broad host-range across several bacterial phyla
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600314/
https://www.ncbi.nlm.nih.gov/pubmed/26453042
http://dx.doi.org/10.1186/s12985-015-0395-0
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