Cargando…
Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600393/ https://www.ncbi.nlm.nih.gov/pubmed/26381016 http://dx.doi.org/10.14814/phy2.12553 |
_version_ | 1782394420652408832 |
---|---|
author | Do, Mai-Khoi Q Shimizu, Naomi Suzuki, Takahiro Ohtsubo, Hideaki Mizunoya, Wataru Nakamura, Mako Sawano, Shoko Furuse, Mitsuhiro Ikeuchi, Yoshihide Anderson, Judy E Tatsumi, Ryuichi |
author_facet | Do, Mai-Khoi Q Shimizu, Naomi Suzuki, Takahiro Ohtsubo, Hideaki Mizunoya, Wataru Nakamura, Mako Sawano, Shoko Furuse, Mitsuhiro Ikeuchi, Yoshihide Anderson, Judy E Tatsumi, Ryuichi |
author_sort | Do, Mai-Khoi Q |
collection | PubMed |
description | Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) triggered its expression exclusively at the early differentiation phase. In order to advance this concept, the present study described that transmembrane heparan/chondroitin sulfate proteoglycans syndecan-2, 4 may be the plausible receptor candidates for HGF and FGF2 to signal Sema3A expression. Results showed that mRNA expression of syndecan-2, 4 was abundant (two magnitudes higher than syndecan-1, 3) in early-differentiated myoblasts and their in vitro knockdown diminished the HGF/FGF2-induced expression of Sema3A down to a baseline level. Pretreatment with heparitinase and chondroitinase ABC decreased the HGF and FGF2 responses, respectively, in non–knockdown cultures, supporting a possible model that HGF and FGF2 may bind to heparan and chondroitin sulfate chains of syndecan-2, 4 to signal Sema3A expression. The findings, therefore, extend our understanding that HGF/FGF2-syndecan-2, 4 association may stimulate a burst of Sema3A secretion by myoblasts recruited to the site of muscle injury; this would ensure a coordinated delay in the attachment of motoneuron terminals onto fibers early in muscle regeneration, and thus synchronize the recovery of muscle fiber integrity and the early resolution of inflammation after injury with reinnervation toward functional recovery. |
format | Online Article Text |
id | pubmed-4600393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley & Sons, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-46003932015-10-15 Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts Do, Mai-Khoi Q Shimizu, Naomi Suzuki, Takahiro Ohtsubo, Hideaki Mizunoya, Wataru Nakamura, Mako Sawano, Shoko Furuse, Mitsuhiro Ikeuchi, Yoshihide Anderson, Judy E Tatsumi, Ryuichi Physiol Rep Original Research Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) triggered its expression exclusively at the early differentiation phase. In order to advance this concept, the present study described that transmembrane heparan/chondroitin sulfate proteoglycans syndecan-2, 4 may be the plausible receptor candidates for HGF and FGF2 to signal Sema3A expression. Results showed that mRNA expression of syndecan-2, 4 was abundant (two magnitudes higher than syndecan-1, 3) in early-differentiated myoblasts and their in vitro knockdown diminished the HGF/FGF2-induced expression of Sema3A down to a baseline level. Pretreatment with heparitinase and chondroitinase ABC decreased the HGF and FGF2 responses, respectively, in non–knockdown cultures, supporting a possible model that HGF and FGF2 may bind to heparan and chondroitin sulfate chains of syndecan-2, 4 to signal Sema3A expression. The findings, therefore, extend our understanding that HGF/FGF2-syndecan-2, 4 association may stimulate a burst of Sema3A secretion by myoblasts recruited to the site of muscle injury; this would ensure a coordinated delay in the attachment of motoneuron terminals onto fibers early in muscle regeneration, and thus synchronize the recovery of muscle fiber integrity and the early resolution of inflammation after injury with reinnervation toward functional recovery. John Wiley & Sons, Ltd 2015-09-17 /pmc/articles/PMC4600393/ /pubmed/26381016 http://dx.doi.org/10.14814/phy2.12553 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Do, Mai-Khoi Q Shimizu, Naomi Suzuki, Takahiro Ohtsubo, Hideaki Mizunoya, Wataru Nakamura, Mako Sawano, Shoko Furuse, Mitsuhiro Ikeuchi, Yoshihide Anderson, Judy E Tatsumi, Ryuichi Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title | Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title_full | Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title_fullStr | Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title_full_unstemmed | Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title_short | Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts |
title_sort | transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of hgf and fgf2 on semaphorin 3a expression in early-differentiated myoblasts |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600393/ https://www.ncbi.nlm.nih.gov/pubmed/26381016 http://dx.doi.org/10.14814/phy2.12553 |
work_keys_str_mv | AT domaikhoiq transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT shimizunaomi transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT suzukitakahiro transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT ohtsubohideaki transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT mizunoyawataru transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT nakamuramako transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT sawanoshoko transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT furusemitsuhiro transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT ikeuchiyoshihide transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT andersonjudye transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts AT tatsumiryuichi transmembraneproteoglycanssyndecan24receptorcandidatesfortheimpactofhgfandfgf2onsemaphorin3aexpressioninearlydifferentiatedmyoblasts |