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Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts

Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted...

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Autores principales: Do, Mai-Khoi Q, Shimizu, Naomi, Suzuki, Takahiro, Ohtsubo, Hideaki, Mizunoya, Wataru, Nakamura, Mako, Sawano, Shoko, Furuse, Mitsuhiro, Ikeuchi, Yoshihide, Anderson, Judy E, Tatsumi, Ryuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600393/
https://www.ncbi.nlm.nih.gov/pubmed/26381016
http://dx.doi.org/10.14814/phy2.12553
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author Do, Mai-Khoi Q
Shimizu, Naomi
Suzuki, Takahiro
Ohtsubo, Hideaki
Mizunoya, Wataru
Nakamura, Mako
Sawano, Shoko
Furuse, Mitsuhiro
Ikeuchi, Yoshihide
Anderson, Judy E
Tatsumi, Ryuichi
author_facet Do, Mai-Khoi Q
Shimizu, Naomi
Suzuki, Takahiro
Ohtsubo, Hideaki
Mizunoya, Wataru
Nakamura, Mako
Sawano, Shoko
Furuse, Mitsuhiro
Ikeuchi, Yoshihide
Anderson, Judy E
Tatsumi, Ryuichi
author_sort Do, Mai-Khoi Q
collection PubMed
description Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) triggered its expression exclusively at the early differentiation phase. In order to advance this concept, the present study described that transmembrane heparan/chondroitin sulfate proteoglycans syndecan-2, 4 may be the plausible receptor candidates for HGF and FGF2 to signal Sema3A expression. Results showed that mRNA expression of syndecan-2, 4 was abundant (two magnitudes higher than syndecan-1, 3) in early-differentiated myoblasts and their in vitro knockdown diminished the HGF/FGF2-induced expression of Sema3A down to a baseline level. Pretreatment with heparitinase and chondroitinase ABC decreased the HGF and FGF2 responses, respectively, in non–knockdown cultures, supporting a possible model that HGF and FGF2 may bind to heparan and chondroitin sulfate chains of syndecan-2, 4 to signal Sema3A expression. The findings, therefore, extend our understanding that HGF/FGF2-syndecan-2, 4 association may stimulate a burst of Sema3A secretion by myoblasts recruited to the site of muscle injury; this would ensure a coordinated delay in the attachment of motoneuron terminals onto fibers early in muscle regeneration, and thus synchronize the recovery of muscle fiber integrity and the early resolution of inflammation after injury with reinnervation toward functional recovery.
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spelling pubmed-46003932015-10-15 Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts Do, Mai-Khoi Q Shimizu, Naomi Suzuki, Takahiro Ohtsubo, Hideaki Mizunoya, Wataru Nakamura, Mako Sawano, Shoko Furuse, Mitsuhiro Ikeuchi, Yoshihide Anderson, Judy E Tatsumi, Ryuichi Physiol Rep Original Research Regenerative mechanisms that regulate intramuscular motor innervation are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed an unexplored role of resident myogenic stem cell (satellite cell)-derived myoblasts as a key presenter of a secreted neural chemorepellent semaphorin 3A (Sema3A); hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) triggered its expression exclusively at the early differentiation phase. In order to advance this concept, the present study described that transmembrane heparan/chondroitin sulfate proteoglycans syndecan-2, 4 may be the plausible receptor candidates for HGF and FGF2 to signal Sema3A expression. Results showed that mRNA expression of syndecan-2, 4 was abundant (two magnitudes higher than syndecan-1, 3) in early-differentiated myoblasts and their in vitro knockdown diminished the HGF/FGF2-induced expression of Sema3A down to a baseline level. Pretreatment with heparitinase and chondroitinase ABC decreased the HGF and FGF2 responses, respectively, in non–knockdown cultures, supporting a possible model that HGF and FGF2 may bind to heparan and chondroitin sulfate chains of syndecan-2, 4 to signal Sema3A expression. The findings, therefore, extend our understanding that HGF/FGF2-syndecan-2, 4 association may stimulate a burst of Sema3A secretion by myoblasts recruited to the site of muscle injury; this would ensure a coordinated delay in the attachment of motoneuron terminals onto fibers early in muscle regeneration, and thus synchronize the recovery of muscle fiber integrity and the early resolution of inflammation after injury with reinnervation toward functional recovery. John Wiley & Sons, Ltd 2015-09-17 /pmc/articles/PMC4600393/ /pubmed/26381016 http://dx.doi.org/10.14814/phy2.12553 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Do, Mai-Khoi Q
Shimizu, Naomi
Suzuki, Takahiro
Ohtsubo, Hideaki
Mizunoya, Wataru
Nakamura, Mako
Sawano, Shoko
Furuse, Mitsuhiro
Ikeuchi, Yoshihide
Anderson, Judy E
Tatsumi, Ryuichi
Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title_full Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title_fullStr Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title_full_unstemmed Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title_short Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts
title_sort transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of hgf and fgf2 on semaphorin 3a expression in early-differentiated myoblasts
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600393/
https://www.ncbi.nlm.nih.gov/pubmed/26381016
http://dx.doi.org/10.14814/phy2.12553
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