SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR...

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Autores principales: Lukinavičius, Gražvydas, Blaukopf, Claudia, Pershagen, Elias, Schena, Alberto, Reymond, Luc, Derivery, Emmanuel, Gonzalez-Gaitan, Marcos, D'Este, Elisa, Hell, Stefan W., Wolfram Gerlich, Daniel, Johnsson, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600740/
https://www.ncbi.nlm.nih.gov/pubmed/26423723
http://dx.doi.org/10.1038/ncomms9497
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author Lukinavičius, Gražvydas
Blaukopf, Claudia
Pershagen, Elias
Schena, Alberto
Reymond, Luc
Derivery, Emmanuel
Gonzalez-Gaitan, Marcos
D'Este, Elisa
Hell, Stefan W.
Wolfram Gerlich, Daniel
Johnsson, Kai
author_facet Lukinavičius, Gražvydas
Blaukopf, Claudia
Pershagen, Elias
Schena, Alberto
Reymond, Luc
Derivery, Emmanuel
Gonzalez-Gaitan, Marcos
D'Este, Elisa
Hell, Stefan W.
Wolfram Gerlich, Daniel
Johnsson, Kai
author_sort Lukinavičius, Gražvydas
collection PubMed
description Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
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spelling pubmed-46007402015-10-21 SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy Lukinavičius, Gražvydas Blaukopf, Claudia Pershagen, Elias Schena, Alberto Reymond, Luc Derivery, Emmanuel Gonzalez-Gaitan, Marcos D'Este, Elisa Hell, Stefan W. Wolfram Gerlich, Daniel Johnsson, Kai Nat Commun Article Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging. Nature Pub. Group 2015-10-01 /pmc/articles/PMC4600740/ /pubmed/26423723 http://dx.doi.org/10.1038/ncomms9497 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lukinavičius, Gražvydas
Blaukopf, Claudia
Pershagen, Elias
Schena, Alberto
Reymond, Luc
Derivery, Emmanuel
Gonzalez-Gaitan, Marcos
D'Este, Elisa
Hell, Stefan W.
Wolfram Gerlich, Daniel
Johnsson, Kai
SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title_full SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title_fullStr SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title_full_unstemmed SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title_short SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
title_sort sir–hoechst is a far-red dna stain for live-cell nanoscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600740/
https://www.ncbi.nlm.nih.gov/pubmed/26423723
http://dx.doi.org/10.1038/ncomms9497
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