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Acidic phospholipids govern the enhanced activation of IgG-B cell receptor
B cells that express the isotype-switched IgG-B cell receptor (IgG-BCR) are one of the driving forces for antibody memory. To allow for a rapid memory IgG antibody response, IgG-BCR evolved into a highly effective signalling machine. Here, we report that the positively charged cytoplasmic domain of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600742/ https://www.ncbi.nlm.nih.gov/pubmed/26440273 http://dx.doi.org/10.1038/ncomms9552 |
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author | Chen, Xiangjun Pan, Weiling Sui, Yinqiang Li, Hua Shi, Xiaoshan Guo, Xingdong Qi, Hai Xu, Chenqi Liu, Wanli |
author_facet | Chen, Xiangjun Pan, Weiling Sui, Yinqiang Li, Hua Shi, Xiaoshan Guo, Xingdong Qi, Hai Xu, Chenqi Liu, Wanli |
author_sort | Chen, Xiangjun |
collection | PubMed |
description | B cells that express the isotype-switched IgG-B cell receptor (IgG-BCR) are one of the driving forces for antibody memory. To allow for a rapid memory IgG antibody response, IgG-BCR evolved into a highly effective signalling machine. Here, we report that the positively charged cytoplasmic domain of mIgG (mIgG-tail) specifically interacts with negatively charged acidic phospholipids. The key immunoglobulin tail tyrosine (ITT) in mIgG-tail is thus sequestered in the membrane hydrophobic core in quiescent B cells. Pre-disruption of such interaction leads to excessive recruitment of BCRs and inflated BCR signalling upon antigen stimulation, resulting in hyperproliferation of primary B cells. Physiologically, membrane-sequestered mIgG-tail can be released by antigen engagement or Ca(2+) mobilization in the initiation of B cell activation. Our studies suggest a novel regulatory mechanism for how dynamic association of mIgG-tail with acidic phospholipids governs the enhanced activation of IgG-BCR. |
format | Online Article Text |
id | pubmed-4600742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46007422015-10-21 Acidic phospholipids govern the enhanced activation of IgG-B cell receptor Chen, Xiangjun Pan, Weiling Sui, Yinqiang Li, Hua Shi, Xiaoshan Guo, Xingdong Qi, Hai Xu, Chenqi Liu, Wanli Nat Commun Article B cells that express the isotype-switched IgG-B cell receptor (IgG-BCR) are one of the driving forces for antibody memory. To allow for a rapid memory IgG antibody response, IgG-BCR evolved into a highly effective signalling machine. Here, we report that the positively charged cytoplasmic domain of mIgG (mIgG-tail) specifically interacts with negatively charged acidic phospholipids. The key immunoglobulin tail tyrosine (ITT) in mIgG-tail is thus sequestered in the membrane hydrophobic core in quiescent B cells. Pre-disruption of such interaction leads to excessive recruitment of BCRs and inflated BCR signalling upon antigen stimulation, resulting in hyperproliferation of primary B cells. Physiologically, membrane-sequestered mIgG-tail can be released by antigen engagement or Ca(2+) mobilization in the initiation of B cell activation. Our studies suggest a novel regulatory mechanism for how dynamic association of mIgG-tail with acidic phospholipids governs the enhanced activation of IgG-BCR. Nature Pub. Group 2015-10-06 /pmc/articles/PMC4600742/ /pubmed/26440273 http://dx.doi.org/10.1038/ncomms9552 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chen, Xiangjun Pan, Weiling Sui, Yinqiang Li, Hua Shi, Xiaoshan Guo, Xingdong Qi, Hai Xu, Chenqi Liu, Wanli Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title | Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title_full | Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title_fullStr | Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title_full_unstemmed | Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title_short | Acidic phospholipids govern the enhanced activation of IgG-B cell receptor |
title_sort | acidic phospholipids govern the enhanced activation of igg-b cell receptor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4600742/ https://www.ncbi.nlm.nih.gov/pubmed/26440273 http://dx.doi.org/10.1038/ncomms9552 |
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